Molecular assessment of dhfr/dhps mutations among Plasmodium vivax clinical isolates after introduction of sulfadoxine/pyrimethamine in combination with artesunate in Iran

Autor:	Sakineh Pirahmadi, Navid Dinparast Djadid, Mandana Afsharpad, Sedigheh Zakeri
Přispěvatelé:	Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP), This study was supported by grants (No. 418 to S. Zakeri) from Institut Pasteur of Iran., Malaria and Vector Research Group (MVRG)
Jazyk:	angličtina
Rok vydání:	2012
Předmět:	MESH: Sequence Analysis DNA medicine.medical_treatment Plasmodium vivax Drug Resistance Artesunate DHPS MESH: Tetrahydrofolate Dehydrogenase Iran Polymerase Chain Reaction chemistry.chemical_compound 0302 clinical medicine MESH: Child Haplotype 030212 general & internal medicine MESH: Dihydropteroate Synthase Child MESH: Aged MESH: Middle Aged MESH: Polymorphism Single Nucleotide Chloroquine MESH: Chloroquine Middle Aged MESH: Infant Artemisinins 3. Good health MESH: Plasmodium vivax Drug Combinations Infectious Diseases Pyrimethamine MESH: Young Adult Child Preschool MESH: Drug Resistance [SDV.IMM]Life Sciences [q-bio]/Immunology pvdhfr Polymorphism Restriction Fragment Length Mutations medicine.drug Microbiology (medical) Adult MESH: Mutation Adolescent Sulfadoxine MESH: Pyrimethamine 030231 tropical medicine MESH: DNA Protozoan Biology Microbiology Polymorphism Single Nucleotide 03 medical and health sciences Antimalarials Young Adult parasitic diseases MESH: Artemisinins Genetics medicine Malaria Vivax Humans Molecular Biology Ecology Evolution Behavior and Systematics Aged MESH: Adolescent MESH: Drug Combinations Dihydropteroate Synthase MESH: Humans MESH: Polymorphism Restriction Fragment Length MESH: Child Preschool Infant MESH: Malaria Vivax Plasmodium falciparum MESH: Adult MESH: Polymerase Chain Reaction Sequence Analysis DNA MESH: Haplotypes DNA Protozoan biology.organism_classification Virology MESH: Antimalarials Sulfadoxine/pyrimethamine Tetrahydrofolate Dehydrogenase Sulfadoxine-pyrimethamine (SP) chemistry Haplotypes pvdhps Mutation MESH: Iran MESH: Sulfadoxine
Zdroj:	Infection, Genetics and Evolution Infection, Genetics and Evolution, Elsevier, 2012, 12 (1), pp.38-44. ⟨10.1016/j.meegid.2011.10.003⟩
ISSN:	1567-1348 1567-7257
DOI:	10.1016/j.meegid.2011.10.003⟩
Popis:	International audience; The increasing use of sulfadoxine/pyrimethamine (SP) for treatment of chloroquine-resistant Plasmodium falciparum has resulted in increased exposure of Plasmodium vivax parasites in areas where both species co-exist. In this study, the extent of mutations/haplotypes in pvdhfr and pvdhps was examined using PCR-RFLP methods in 427 P. vivax isolates in Iran after 4 years of introducing SP as the first-line anti-malarial drug in Iran. Mutations were detected in three codons of pvdhfr (F57L, S58R and S117N) and in one of pvdhps (A383G) and the majority of isolates had double mutations (58R/117N, 45.4%). In addition, the frequency of 57L mutation was detected in 8.2% of P. vivax isolates. This frequency was significantly increased when compared with a similar study on P. vivax isolates in 2005 (X(2) test, P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba34e190cf909bd2534822a7cc31e535 https://hal-riip.archives-ouvertes.fr/pasteur-00750730 Zobrazit plný text záznamu Full Text from ScienceDirect