In vitro evaluation of inhalable isoniazid-loaded surfactant liposomes as an adjunct therapy in pulmonary tuberculosis

Autor: Rangan Banerjee, G. Chimote
Rok vydání: 2010
Předmět:
Pathology
Antitubercular Agents
Biocompatible Materials
Antimycobacterial
chemistry.chemical_compound
Mice
Drug Delivery Systems
Pulmonary surfactant
Drug Stability
Materials Testing
Dppc
Lung
Complement Activation
Liposome
Drug Carriers
respiratory system
medicine.anatomical_structure
Drug-Delivery System
Drug delivery
Infection
Drug carrier
Infants
medicine.medical_specialty
Materials science
Biocompatibility
1
2-Dipalmitoylphosphatidylcholine
medicine.drug_class
Surface Properties
Drug Compounding
Biomedical Engineering
Microbial Sensitivity Tests
Respiratory-Distress Syndrome
Cell Line
Biomaterials
Pulmonary Tuberculosis
medicine
Isoniazid
Animals
Humans
Particle Size
Aerosol
Tuberculosis
Pulmonary
Rifampicin
Chromatography
Nebulizers and Vaporizers
technology
industry
and agriculture
Pulmonary Surfactants
chemistry
Dipalmitoylphosphatidylcholine
Liposomes
Adsorption
Pulmonary Surfactant
Zdroj: Journal of biomedical materials research. Part B, Applied biomaterials. 94(1)
ISSN: 1552-4981
Popis: In this study, exogenous pulmonary surfactant was evaluated as an inhalable drug carrier for antitubercular drug isoniazid (INH). Isoniazid-entrapped liposomes of dipalmitoyl-phosphatidylcholine (DPPC) (the most abundant lipid of lung surfactant and exogenous surfactant) were developed and evaluated for size, drug entrapment, release, in vitro alveolar deposition, biocompatibility, antimycobacterial activity, and pulmonary surfactant action. Isoniazid-entrapped DPPC liposomes were about 750 nm in diameter and had entrapment efficiency of 36.7% +/- 1.8%. Sustained release of INH from DPPC liposomes was observed over 24 h. In vitro alveolar deposition efficiency using the twin impinger exhibited similar to 25-27% INH deposition in the alveolar chamber upon one minute nebulization using a jet nebulizer. At 37 degrees C, the formulation had better pulmonary surfactant function with quicker reduction of surface tension on adsorption (36.7 +/- 0.4 mN/m) than DPPC liposomes (44.7 +/- 0.6 mN/m) and 87% airway patency was exhibited by the formulation in a capillary surfactometer. The formulation was biocompatible and had antimycobacterial activity. The isoniazid-entrapped DPPC liposomes could fulfill the dual purpose of pulmonary drug delivery and alveolar stabilization due to antiatelectatic effect of the surfactant action which can improve the reach of antitubercular drug INH to the alveoli. (C) 2010 . J Biomed Mater Res Part B: Appl Biomater 946: 1-10, 2010.
Databáze: OpenAIRE
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