Proportion of HIV-1 Infected CD8+CD4- T Lymphocytes In Vivo
| Autor: | Nancy Lamoureux, Karen F.T. Copeland, Naveed Gulzar, Mary-Ellen Arsenault, Bilge Diker, Jose Deschatelets, John E. Kim, Donald W. Cameron, Jennifer Mihowich |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes T cell Population Human immunodeficiency virus (HIV) HIV Infections CD8-Positive T-Lymphocytes Biology medicine.disease_cause Pathogenesis Viral Proteins In vivo Virology medicine Humans education education.field_of_study Reverse Transcriptase Polymerase Chain Reaction Middle Aged Flow Cytometry Molecular biology Infectious Diseases medicine.anatomical_structure HIV-1 biology.protein RNA Viral Antibody Ex vivo CD8 |
| Zdroj: | Current HIV Research. 6:585-596 |
| ISSN: | 1570-162X |
| DOI: | 10.2174/157016208786501544 |
| Popis: | The proportion and significance of HIV-1 infection of CD8+ T-cells was examined in a patient cohort of HIV-1 seropositive (n=28) and seronegative individuals (n=4). It was hypothesized that irrespective of the clinical status of the patients, productively HIV-1 infected CD8+ T-cells would be found and these cells would contribute to the plasma viral load in vivo. Flow cytometric analysis using fluorochrome-conjugated antibodies, RT-PCR analysis using HIV-1(pol) specific primers, and quantification of HIV-1 viral transcripts by ex vivo culture of isolated CD8+ T-cells were employed. In 22 of the 28 patient samples analyzed, a significantly higher proportion of cells with expression of CD8+HIV-1(gag)+ than of CD4+HIV-1(gag)+ T-cells was observed (36.9% +/- 10.0% vs 26.4% +/- 13.1% respectively, p0.01). No correlation was observed between absolute CD4 counts, CD8 counts, plasma viral load and CD8+ T cell infection. RT-PCR analysis indicated the presence of HIV-1 transcripts in the ex vivo isolated CD8+ T-cell population. Ex vivo isolated CD8+ T-cells demonstrated productive infection over time. We conclude, with three lines of evidence detecting and measuring HIV-1 infection of CD8+ T-lymphocytes, that this cellular target and reservoir may be central to HIV-1 pathogenesis. |
| Databáze: | OpenAIRE |
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