Cardioprotection Afforded by St Thomas Solution Is Enhanced by Emulsified Isoflurane in an Isolated Heart Ischemia Reperfusion Injury Model in Rats
| Autor: | Jin Liu, Chen Yanfang, Tao Li, Han Huang, Wensheng Zhang, Shan-ling Liu |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
Fat Emulsions Intravenous medicine.medical_specialty Myocardial Infarction Ischemia Therapeutics Sodium Chloride Potassium Chloride Rats Sprague-Dawley Calcium Chloride Random Allocation Blind study Internal medicine Emulsified isoflurane medicine Animals Creatine Kinase MB Form Magnesium Cardioplegic Solutions Cardioprotection Isoflurane business.industry Hemodynamics Heart Isolated heart medicine.disease Rats Bicarbonates Disease Models Animal Anesthesiology and Pain Medicine Reperfusion Injury Anesthesia Anesthetics Inhalation Cardiology Thomas' solution Cardiology and Cardiovascular Medicine business Reperfusion injury medicine.drug |
| Zdroj: | Journal of Cardiothoracic and Vascular Anesthesia. 24:99-103 |
| ISSN: | 1053-0770 |
| DOI: | 10.1053/j.jvca.2009.10.016 |
| Popis: | The purpose of this study was to investigate whether adding emulsified isoflurane to St Thomas cardioplegia solution could enhance the cardiac protection after cardioplegic arrest in rats.A randomized, blind study.A university laboratory.Thirty male Sprague-Dawley rats.Thirty isolated heart preparations were randomly divided into 3 groups (n = 10/group) according to the different cardioplegia solutions being given: St Thomas solution mixed with emulsified isoflurane (containing 2.8% of isoflurane, group EI), St Thomas solution mixed with emulsified Intralipid (Huarui Pharmacy, Wuxi, Jiangsu, China) (group EL), and St Thomas solution alone (group St). In the 35-minute normothermic ischemia period, infusion of cardioplegia solution was repeated every 15 minutes. After the 35-minute ischemia period, the heart was perfused with Krebs-Henseleit buffer for another 2 hours.The functional parameters of the heart were monitored throughout the experiments. The coronary effluent was collected for measuring the activity of CK-MB 30 minutes after reperfusion, and the infarct size was assessed at the end of reperfusion. The infarct size in group EI (24% +/- 4%) was reduced when compared with that in group EL (31% +/- 8%, p0.05) and group St (43% +/- 9%, p0.001). The CK-MB activity in group EI was decreased significantly when compared with that in group EL and group St (p0.05). The functional recovery in group EI also was improved. Compared with standard St Thomas solution alone, adding 30% Intralipid alone also significantly reduced the infarct size and the CK-MB leakage and improved the recovery of the mechanical function.St Thomas cardioplegia solution supplemented with emulsified isoflurane enhanced its cardioprotection in an isolated heart ischemia reperfusion injury model in rats. |
| Databáze: | OpenAIRE |
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