Alteration of the passive electrical properties of adriamycin-treated red cell membrane deduced from dielectric spectroscopy

Autor:	Annarica Calcabrini, G. Isacchi, Federico Bordi, Cesare Cametti, Marco Diociaiuti, Giuseppe Arancia, Agnese Molinari
Rok vydání:	1991
Předmět:	Permittivity Chemistry Analytical chemistry Biophysics Dielectric Conductivity law.invention Dielectric spectroscopy Membrane law Membrane fluidity Electrochemistry Electron microscope Physical and Theoretical Chemistry Lipid bilayer
Zdroj:	Bioelectrochemistry and Bioenergetics. 26:177-192
ISSN:	0302-4598
DOI:	10.1016/0302-4598(91)80022-u
Popis:	The interaction of adriamycin (ADR) molecules with human erythrocytes has been investigated by radiowave dielectric spectroscopy complemented with electron microscopy methods to elucidate the extent of modification induced by this antitumoural drug in the passive electrical properties of the membrane. The electrical parameters, i.e. the membrane conductivity σs and the membrane permittivity ϵs, deduced by analysis of the conductivity dispersion curves at radiowave frequencies demonstrate that ADR molecule is incorporated in the lipid bilayer, modifying the transport properties of the overall membrane. Moreover, a two-shell dielectric model provides further indication that the most relevant alterations occur in the innermost layer of the membrane. This finding is shown to be fully consistent with the observed ultrastructural alterations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba3163c820940dc164be186205a7f6b2 https://doi.org/10.1016/0302-4598(91)80022-u Zobrazit plný text záznamu