Assessment of proliferative activity by AgNOR and PCNA in prostatic tissues of ram lambs implanted with zeranol
| Autor: | Hayrettin Okut, T Guvenc, Hayati Yüksel, Mustafa Yavuz Gülbahar |
|---|---|
| Přispěvatelé: | Ondokuz Mayıs Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Male
Pathology medicine.medical_specialty Silver Staining Prostatic Hyperplasia Sheep Diseases Biology Cholangiocyte chemistry.chemical_compound Random Allocation Endocrinology Acinus Prostate Proliferating Cell Nuclear Antigen medicine Zeranol Nucleolus Organizer Region Animals Estrogens Non-Steroidal Drug Implants Sheep Dose-Response Relationship Drug Staining and Labeling Immunohistochemistry Proliferating cell nuclear antigen Dose–response relationship medicine.anatomical_structure chemistry biology.protein Animal Science and Zoology Nucleolus organizer region Immunostaining Cell Division Biotechnology |
| DOI: | 10.1111/j.1439-0531.2005.00604.x |
| Popis: | Guvenc, Tolga/0000-0003-1468-3415; GULBAHAR, MUSTAFA YAVUZ/0000-0001-8268-7659 WOS: 000231972600010 PubMed: 16149954 The aim of this study was to assess cellular proliferation using silver-stained nucleolar organizer regions (AgNOR) and the proliferating cell nuclear antigen (PCNA) in various tissues in the prostate of ram lambs implanted with increasing zeranol doses and to compare the sensitivity of different tissues of lamb prostate to zeranol. Twenty-four Akkaraman lambs were implanted with increasing zeranol doses, including 12 mg (n = 8), 24 mg (n = 8) and 96 mg (n = 8), with eight lambs serving as controls. After 33 days, the prostate tissues of the lambs were stained using AgNOR and PCNA techniques. The prostate tissues were divided into two compartments - the epithelial tissues, including glandular acinus, collecting duct and penile urethra, and the non-epithelia] tissues.. including interstitial tissue and striated muscle. AgNOR dots and PCNA index on each prostatic tissue were counted under a light microscope and were evaluated statistically. AgNOR staining in the treatment groups showed a higher score in the non-epithelial tissues than the epithelial components, whereas the PCNA index was significant in the epithelia] tissues and non-epithelial, tissues had very low. PCNA immunostaining. According to the PCNA index, collecting duct epithelium showed more sensitivity to increasing zeranol doses and according to AgNOR counts, there was no difference of sensitivity to zeranol among tissues of the same origin. Both AgNOR counts and PCNA indexes seem to be valuable proliferating markers for the epithelial components of ram prostate, but PCNA index had no significance in relation to the non-epithelial components in contrast to AgNOR counts. Therefore, the controversial results arising from the combined use of both techniques as proliferating markers for the ram prostate should be considered in further studies. |
| Databáze: | OpenAIRE |
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