Analysis of the phytochrome gene family in Ceratodon purpureus by gene targeting reveals the primary phytochrome responsible for photo- and polarotropism
| Autor: | Sven Dienstbach, Andrea Weisert, Christoph Forreiter, Franz Mittmann |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Chlorophyll
Light Plant Science Bryophyta Biology chemistry.chemical_compound Gene Knockout Techniques Genetics Gene family Phototropism Gene Phylogeny Ceratodon purpureus Phytochrome Reverse Transcriptase Polymerase Chain Reaction Gene targeting biology.organism_classification Blotting Southern chemistry Multigene Family Gene Targeting Mutation Homologous recombination |
| Zdroj: | Planta. 230(1) |
| ISSN: | 1432-2048 |
| Popis: | Using gene targeting by homologous recombination in Ceratodon purpureus, we were able to knock out four phytochrome photoreceptor genes independently and to analyze their function with respect to red light dependent phototropism, polarotropism, and chlorophyll content. The strongest phenotype was found in knock-out lines of a newly described phytochrome gene termed CpPHY4 lacking photo- and polarotropic responses at moderate fluence rates. Eliminating the atypical phytochrome gene CpPHY1, which is the only known phytochrome-like gene containing a putative C-terminal tyrosine kinase-like domain, affects red light-induced chlorophyll accumulation. This result was surprising, since no light dependent function was ever allocated to this unusual gene. |
| Databáze: | OpenAIRE |
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