Quenching by sodium thiosulfate does not influence 16S rRNA gene sequencing profiles of reclaimed water from three sites in the Mid-Atlantic, United States
| Autor: | Joseph Haymaker, Mary Theresa Callahan, Fawzy Hashem, Derek Foust, Eoghan M. Smyth, Hillary A. Craddock, Emmanuel F. Mongodin, Anthony Bui, Rico Duncan, Eric B. May, Sultana Solaiman, Amy R. Sapkota, Shirley A. Micallef, Sarah M. Allard |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Thiosulfates
010501 environmental sciences Sodium thiosulfate 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine RNA Ribosomal 16S 030212 general & internal medicine Mid-Atlantic Region RRNA processing 0105 earth and related environmental sciences General Environmental Science Quenching Microbiota High-Throughput Nucleotide Sequencing Water Biodiversity 16S ribosomal RNA Reclaimed water chemistry 16s rrna gene sequencing Sample collection Water Microbiology |
| Zdroj: | Environmental Research. 172:296-300 |
| ISSN: | 0013-9351 |
| Popis: | A quenching agent is commonly added to chlorinated, reclaimed water during sample collection to prevent chlorine-mediated die-off of viable microbiota. However, the effect of quenching on downstream 16S rRNA-based bacterial community analyses is unclear. We conducted a side-by-side comparison of 16S rRNA sequencing data from reclaimed water samples quenched with sodium thiosulfate and non-quenched samples. Our data showed that 16 S rRNA processing and sequencing methods, and resulting bacterial profiles, were not negatively impacted by quenching. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect