Comparative Evaluation of Four Phenotypic Tests for Detection of Carbapenemase-Producing Gram-Negative Bacteria
| Autor: | Pierre Bogaerts, Audrey Noël, Sami Yunus, Te-Din Huang, Catherine Berhin, Martin Hoebeke, Warda Bouchahrouf, Youri Glupczynski |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Bacilli Gram-negative bacteria 030106 microbiology Sensitivity and Specificity beta-Lactamases Microbiology Comparative evaluation 03 medical and health sciences 0302 clinical medicine Bacterial Proteins Enterobacteriaceae Pseudomonas parasitic diseases Humans Mass Screening 030212 general & internal medicine Prospective Studies Mass screening Bacteriological Techniques biology Acinetobacter Bacteriology Carbapenemase producing biology.organism_classification Gram-Negative Bacterial Infections |
| Popis: | Four screening assays aimed for rapid detection of carbapenemase production from Gram-negative bacterial isolates, i.e., the Neo-Rapid Carb kit (Rosco Diagnostica A/S), the Rapidec Carba NP test (bioMérieux SA), the β Carba test (Bio-Rad Laboratories N.V.), and a homemade electrochemical assay (BYG Carba test) were evaluated against a panel comprising 328 clinical isolates ( Enterobacteriaceae [ n = 198] and nonfermentative Gram-negative bacilli [ n = 130]) with previously characterized resistance mechanisms to carbapenems. Among Enterobacteriaceae isolates, the BYG Carba test and the β Carba test showed excellent sensitivities (respectively, 100% and 97.3%) and specificities (respectively, 98.9% and 97.7%). The two other assays yielded poorer performances with sensitivity and specificity of 91.9% and 83.9% for the Rapidec Carba NP test and of 89.2% and 89.7% for the Neo-Rapid Carb kit, respectively. Among Pseudomonas spp., sensitivities and specificities ranged, respectively, from 87.3% to 92.7% and from 88.2% to 94.1%. Finally, all tests performed poorly against Acinetobacter spp., with sensitivities and specificities, respectively, ranging from 27.3% to 75.8% and from 75 to 100%. Among commercially available assays, the β Carba test appeared to be the most convenient for routine use and showed the best overall performances, especially against OXA-48-like producers. The excellent performance of the BYG Carba test against Enterobacteriaceae was confirmed (100% sensitivity and 98.9% specificity). |
| Databáze: | OpenAIRE |
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