Fiber density of collagen grafts impacts rabbit urethral regeneration
| Autor: | Kalitha Pinnagoda, Elif Vardar, E.M. Engelhardt, Ganesh Vythilingam, Tunku Kamarul, Peter Frey, S. Sothilingam, Rajendrarao C. Thambidorai, Jeffrey A. Hubbell, Hans M. Larsson |
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| Předmět: |
Dietary Fiber
Male Reconstructive surgery medicine.medical_specialty 030232 urology & nephrology lcsh:Medicine Transplants Article Extracellular matrix 03 medical and health sciences 0302 clinical medicine Urethra medicine Animals Regeneration lcsh:Science Collagen/metabolism Extracellular Matrix Models Animal Muscle Smooth Rabbits Reconstructive Surgical Procedures Tissue Engineering/methods Transplants/metabolism Transplants/surgery Urethra/pathology Urethra/transplantation Multidisciplinary Tissue Engineering business.industry Regeneration (biology) lcsh:R Rabbit (nuclear engineering) Histology Plastic Surgery Procedures Surgery Bridge (graph theory) medicine.anatomical_structure surgical procedures operative 030220 oncology & carcinogenesis lcsh:Q Collagen Fiber density business |
| Zdroj: | Scientific reports, vol. 8, no. 1, pp. 10057 Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-8 (2018) |
| Popis: | There is a need for efficient and “off-the-shelf” grafts in urethral reconstructive surgery. Currently available surgical techniques require harvesting of grafts from autologous sites, with increased risk of surgical complications and added patient discomfort. Therefore, a cost-effective and cell-free graft with adequate regenerative potential has a great chance to be translated into clinical practice. Tubular cell-free collagen grafts were prepared by varying the collagen density and fiber distribution, thereby creating a polarized low fiber density collagen graft (LD-graft). A uniform, high fiber density collagen graft (HD-graft) was engineered as a control. These two grafts were implanted to bridge a 2 cm long iatrogenic urethral defect in a rabbit model. Histology revealed that rabbits implanted with the LD-graft had a better smooth muscle regeneration compared to the HD-graft. The overall functional outcome assessed by contrast voiding cystourethrography showed patency of the urethra in 90% for the LD-graft and in 66.6% for the HD-graft. Functional regeneration of the rabbit implanted with the LD-graft could further be demonstrated by successful mating, resulting in healthy offspring. In conclusion, cell-free low-density polarized collagen grafts show better urethral regeneration than high-density collagen grafts. |
| Databáze: | OpenAIRE |
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