Prevalence of Cytomegalovirus Infection in Patients With Ulcerative Colitis by Using PCR Technique
| Autor: | Farzad Khalily, Masood Ghane, Sahar Mehrabani-Khasraghi, Mitra Ameli |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
medicine.diagnostic_test business.industry Incidence (epidemiology) lcsh:R Congenital cytomegalovirus infection lcsh:Medicine medicine.disease Malignancy Inflammatory bowel disease DNA extraction Gastroenterology Ulcerative colitis law.invention law Internal medicine Biopsy Medicine business Polymerase chain reaction |
| Zdroj: | Majallah-i taḥqīqāt-i ̒ulūm-i pizishkī-i Zāhidān, Vol 17, Iss 11, Pp 1-4 (2015) |
| ISSN: | 2228-6403 |
| Popis: | Background: Ulcerative colitis is one type of inflammatory bowel disease. Objectives: The aim of this study was to investigate the prevalence of cytomegalovirus in patients with ulcerative colitis in comparison with healthy subjects by using PCR technique. Patients and Methods: In this analytical case-control study, 5 patients with ulcerative colitis and 30 patients without malignancy as controls were selected. Biopsy specimens were frozen under sterile conditions at -20oC. After DNA extraction, analysis of polymerase chain reaction (PCR) to detect CMV DNA in tissue samples was performed. Statistical analysis was performed with χ2 test. Results: CMV DNA was found in 80% of ulcerative colitis samples (4 of 5) and 30% of the non-malignant control group (9 of 30). Statistical analysis showed significant association between the prevalence of CMV and incidence ulcerative colitis in comparison with the control group (P = 0.03). Conclusions: The results of the present study has shown ulcerative colitis patients have a predisposition to CMV infection as compared to healthy individuals, but the etiologic link between ulcerative colitis and CMV infection needs to be studied. |
| Databáze: | OpenAIRE |
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