Genetic diversity of theHwp1gene andHIS3, EF3, CDC3microsatellites and antifungal susceptibility profiles ofCandida albicansisolates from Yaoundé HIV-infected patients
| Autor: | Pascal Drakulovski, Eric Delaporte, Sébastien Bertout, Fabrice Fekam Boyom, Charles Kouanfack, Jacques Reynes, Thierry Kammalac Ngouana, Donika Krasteva, Michèle Mallié, Rufin M.K. Toghueo |
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| Přispěvatelé: | Recherches Translationnelles sur le VIH et les maladies infectieuses ( TransVIHMI ), Université Montpellier 1 ( UM1 ) -Université Cheikh Anta Diop ( UCAD ) -Universtié Yaoundé 1 (Cameroun)-Université de Montpellier ( UM ), Laboratoire de Parasitologie-Mycologie, Montpellier ( MIVEGEC ), Université Montpellier 1 ( UM1 ) -Université Montpellier 2 - Sciences et Techniques ( UM2 ) -Centre Hospitalier Régional Universitaire [Montpellier] ( CHRU Montpellier ) -Institut de recherche pour le développement [IRD] : UR224-Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Département Maladies Infectieuses et Tropicales, Hôpital Universitaire, Montpellier, France, Centre Hospitalier Régional Universitaire [Montpellier] ( CHRU Montpellier ), Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques er émergentes (TransVIHMI), Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Institut de Recherche pour le Développement (IRD)-Université de Yaoundé I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antifungal Agents Genotype Genes Fungal 030106 microbiology HIV Infections Yaoundé Microbial Sensitivity Tests Biology Real-Time Polymerase Chain Reaction Hwp1 microsatellites law.invention Microbiology Fungal Proteins 03 medical and health sciences Minimum inhibitory concentration [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Drug Resistance Fungal law Candida albicans Humans Cameroon Genotyping [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology [ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology Polymerase chain reaction 2. Zero hunger Genetics Molecular Epidemiology [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health Membrane Glycoproteins Molecular epidemiology Broth microdilution Genetic Variation General Medicine biology.organism_classification Corpus albicans 3. Good health Molecular Typing [ SDV.MHEP.MI ] Life Sciences [q-bio]/Human health and pathology/Infectious diseases Infectious Diseases [ SDV.BA.MVSA ] Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health antifungal Microsatellite Repeats |
| Zdroj: | Medical Mycology Medical Mycology, Oxford University Press, 2017, 55 (5), pp.546-554. 〈10.1093/mmy/myw108〉 Medical Mycology, Oxford University Press, 2017, 55 (5), pp.546-554. ⟨10.1093/mmy/myw108⟩ |
| ISSN: | 1460-2709 1369-3786 1365-280X |
| Popis: | International audience; The molecular epidemiology and the antifungal susceptibility profiles of Candida albicans are scarce in Cameroon. Authors studied the genetic diversity and the antifungal susceptibility of C. albicans isolates from Yaoundé HIV-infected patients. Clinical isolates were obtained by mycological diagnosis of oropharyngeal swabs, stools, urine, and vaginal swabs from patients. C. albicans isolates were confirmed by the Light cycler real-time PCR of the ITS1 region of the 5.8s ribosomal DNA. The ABC genotypes and the Hwp1 gene amplification were carried out with specific primers. Microsatellite length polymorphism of HIS3, CDC3, and EF3 microsatellites was analysed. The antifungal susceptibility testing was carried out by the CLSI broth microdilution M27-A3 and M27-S4 protocols. The minimal inhibitory concentration (MIC) results were interpreted according to updated clinical breakpoints (CBPs) recommended by the CLSI or epidemiological cut-off values (ECVs). One hundred and thirteen (113) isolates were obtained from the analysis of 1218 samples. The ABC genotyping showed 79 (69.91%) genotype A, 24 (21.23%) genotype B, and 10 (8.84%) genotype C. The Hwp1 gene amplification provided a newly observed genetic polymorphism, named H and 5 genotypes described (H1-H5). The microsatellite analysis generated 65 molecular types. All the isolates were susceptible to amphotericin B (MIC ≤ 1 μg/ml); 79.64% of isolates were wild type to itraconazole (MIC ≤ 0.12 μg/ml); and 86.72% of isolates were susceptible to fluconazole (MIC ≤ 2 μg/ml). These results highlight the important genetic diversity of C. albicans isolates among Yaoundé HIV-infected patients and bring clues for the comprehension of the molecular epidemiology of the yeast in Cameroon. |
| Databáze: | OpenAIRE |
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