Ia-like antigen expression on biologically different human melanoma cell lines
| Autor: | Max Reynier, Catherine Rohner, Christian Aubert, Jorge Kalil, Solange Lebec |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Adult
Male medicine.drug_class Radioimmunoassay Biology Cross Reactions Monoclonal antibody Cell Line Antigen medicine Humans Lymph node Melanoma HLA-D Antigens Beta-2 microglobulin Histocompatibility Antigens Class II Antibodies Monoclonal Middle Aged medicine.disease Molecular biology Superficial spreading melanoma medicine.anatomical_structure Oncology Cell culture Female beta 2-Microglobulin |
| Zdroj: | European journal of cancerclinical oncology. 20(5) |
| ISSN: | 0277-5379 |
| Popis: | Ia-like antigen binding of a large panel of monoclonal antibodies (six anti-human Ia-like monoclonal antibodies and ten murine anti-Ia monoclonal antibodies cross-reactive with human Ia-like antigens) were compared on seven permanent human melanoma cell lines by radioimmunoassay. Cell lines were initiated from primary or metastatic tumors and presented various levels of tumorigenicity (assessed by heterotransplantation in nude mice) and pigmentation (shown by 5-S-cysteinyldopa determination and cytological data). Two cell lines originated from the same primary melanoma, while two other pairs of cell lines originated from superficial spreading melanoma or metastatic lymph node of the same patients. Identical Ia-like allodeterminants were found in cell lines of the same individual origin. Quantitative expression of β 2 -microglobulin and Ia-like antigens was similar in all cell lines except for one, in which these molecules were expressed in lower amounts. These results indicate that Ia-like antigen expression of the cell lines is unrelated to primary or metastatic origin, degree of pigmentation and ability to grow in nude mice. |
| Databáze: | OpenAIRE |
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