Tuning Alginate-Gelatin Bioink Properties by Varying Solvent and Their Impact on Stem Cell Behavior
| Autor: | Sha Huang, Xiaobing Fu, Zhao Li, Tian Hu, Yufan Liu, Haigang Shi, Jiangfan Xie, Bin Yao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
food.ingredient Lineage differentiation Alginates Cell Survival lcsh:Medicine Mice Transgenic 02 engineering and technology Gelatin Article law.invention 03 medical and health sciences Mice food law Materials Testing medicine Animals lcsh:Science Cells Cultured Cell Aggregation Cell Proliferation Mechanical property 3D bioprinting Multidisciplinary Tissue Scaffolds Chemistry Stem Cells lcsh:R Bioprinting Cell Differentiation 021001 nanoscience & nanotechnology Embryo Mammalian Solvent Mice Inbred C57BL 030104 developmental biology Ionic strength Calibration Printing Three-Dimensional Biophysics Solvents Ink lcsh:Q Stem cell Swelling medicine.symptom 0210 nano-technology Rheology |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-8 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Bioink optimization is considered as one of main challenges in cell-laden 3D bioprinting. Alginate-Gelatin (Alg-Gel) hydrogel have been extensively used as bioink. However, its properties could be influenced by various parameters, and little is known about the evidence featuring the impact of solvent. Here we investigated four Alg-Gel bioink by varying solvent ionic strength (named B-1, B-2, B-3 and B-4). Mechanical properties and printability of bioink samples and their impacts on behaviors of encapsulated epidermal stem cells (ESCs) were tested. Bioink with increased ionic strength of solvent showed decreased stiffness and viscosity, and increased swelling and degradation by printability and mechanical property tests. Due to the increased swelling and degradation was associated with shape-maintenance of post-printing constructs, B-3 and B-4 were hardly observable after 14 days. Cellular behaviors were assessed through viability, proliferation, aggregation and differentiation tests. B-2 with optimal properties resulted in higher viability and proliferation of ESCs, and further facilitated cellular aggregation and lineage differentiation. We demonstrated that the solvent can be tuned by ionic strength to control the properties of Alg-Gel bioink and post-printing constructs, which represented a promising avenue for promotion of therapeutic stem cell behaviors in 3D bioprinting. |
| Databáze: | OpenAIRE |
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