A contingent replication assay for the detection of protein-protein interactions in animal cells
| Autor: | Zhen Xi Wang, Haren A. Vasavada, Sherman M. Weissman, F. J. Germino, Subinay Ganguly |
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| Rok vydání: | 1991 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
DNA Replication Saccharomyces cerevisiae Proteins Antigens Polyomavirus Transforming Recombinant Fusion Proteins Simian virus 40 Biology Transfection DNA-binding protein Cell Line Protein–protein interaction Fungal Proteins Viral Proteins Complementary DNA Animals Genomic library Enhancer Gene Library Fungal protein Multidisciplinary cDNA library DNA replication Proteins Herpes Simplex Virus Protein Vmw65 Molecular biology Cell biology DNA-Binding Proteins Repressor Proteins Genetic Techniques Plasmids Transcription Factors Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 88:10686-10690 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.88.23.10686 |
| Popis: | We have developed a sensitive and rapid assay system termed the contingent replication assay (CRA) for selecting cDNAs with desired functional properties from a cDNA library. The system functions in animal cells and permits enrichment of the desired cDNA in small-scale and convenient experiments. The assay can be used for the enrichment of proteins that activate transcription from conditional enhancers, bind to specific DNA sequences, or interact with target proteins of interest. In this communication we report the application of this assay to study protein-protein interactions in animal cells. |
| Databáze: | OpenAIRE |
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