Diagnostic accuracy of combinations of serological biomarkers for identifying clinical tuberculosis
| Autor: | Araujo, Z., Macias-Segura, N., López-Ramos, J.E., de Waard, J.H., Vanegas, M., Patarroyo, M.A., Salgado, A., Enciso-Moreno, J.A., Araujo, Zaida, Macias-Segura, Noe, López-Ramos, Juan E., de Waard, Jacobus H., Vanegas, Magnolia, Patarroyo, Manuel A., Salgado, Antonio, Enciso-Moreno, Jose Antonio |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Biología 0403 veterinary science Immunoreactivity 0302 clinical medicine Enzyme Linked Immunosorbent Assay Medicine Area Under The Curve Sputum Analysis Subclinical infection Staining Predictive Value Early Secretory Antigenic Target 6 biology Latent tuberculosis Cd14 Antigen 04 agricultural and veterinary sciences General Medicine Gelatinase B Infectious Diseases Delayed hypersensitivity Urokinase Receptor Diagnostic Accuracy Biomarker (medicine) Female Diagnostic Test Accuracy Study Human Adult Tuberculosis 040301 veterinary sciences Major Clinical Study 030231 tropical medicine Tuberculin Bacterial antigen Microbiology Article X Ray Amino acid sequence Mycobacterium tuberculosis Unclassified Drug 03 medical and health sciences Anti P 29878 Latent Tuberculosis Virology Synthetic Peptide Controlled Study Anti P 12033 Receiver Operating Characteristic Anti P 12034 Peptide Antibody Tuberculin Test business.industry Anti P 12037 Blood Examination Clinical Feature biology.organism_classification medicine.disease Thorax Radiography Immunoglobulin G Biological Marker Immunology Bacterium Culture Delayed Hypersensitivity Parasitology Cxcl9 Chemokine business Mycobacterium Tuberculosis Sensitivity And Specificity Marcadores bioquímicos |
| Zdroj: | (2016) Global Tuberculosis Report 2016, http://www.who.int/tb/publications/global_report/en/, Accessed:12 October 2016) Repositorio EdocUR-U. Rosario Universidad del Rosario instacron:Universidad del Rosario |
| ISSN: | 1972-2680 |
| Popis: | Introduction: Confirmation of tuberculosis (TB) cases in endemic TB settings is a challenge; obtaining fast and cheap, though accurate, diagnostic tools such as biomarkers is thus urgently needed to enable the early detection of TB. This paper evaluates the diagnostic accuracy of combinations of host serological biomarkers for identifying TB. Methodology: Enzyme-linked immunosorbent assays (ELISA) were used on 70 Venezuelan Creole individuals for evaluating host biomarkers (i.e. CXCL9, sCD14, MMP9 and uPAR proteins) and anti-synthetic peptides covering certain Mycobacterium tuberculosis (Mtb) ESAT-6 (P-12033, P-12034 and P-12037) and Ag85A (P-29878) antigen sequences. The target population consisted of adults having active TB (ATB, n = 28), the tuberculin skin test positive (TST+) or individuals with latent TB infection (LTB, n = 28) and TST- or control subjects (CTRL, n = 14). Results: Receiver operator curve (ROC) analysis revealed good biosignature discriminative ability for 5 serological biomarkers; the accuracy of 3 combinations had a good discriminative ability for diagnosing TB. Anti-P-12034/uPAR detected TB with 96.7% sensitivity and 86.0% specificity, followed by anti-P-12033/uPAR having 96.7% sensitivity and 81.4% specificity. Anti-P-29878/MMP9 had the highest sensitivity (100%), but low specificity (52.17%). Biomarker combinations did not prove efficacious for identifying incipient subclinical TST+TB− subjects at high-risk for TB. Conclusions: The anti-P-12034/uPAR combination could be useful for identifying clinical TB patients. Such an approach holds promise for further validation. |
| Databáze: | OpenAIRE |
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