Allergen-Specific Immunotherapy Alters the Frequency, as well as the FcR and CLR Expression Profiles of Human Dendritic Cell Subsets

Autor: Frida Rydnert, Sissela Broos, Malin Lindstedt, Morgan Andersson, Lennart Greiff, Kristina Lundberg
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
Physiology
medicine.medical_treatment
Thrombomodulin
lcsh:Medicine
Cell Count
Plant Science
Receptors
Fc
Immunoglobulin E
Immune tolerance
White Blood Cells
0302 clinical medicine
Spectrum Analysis Techniques
Animal Cells
Antibody Specificity
Allergies
Medicine and Health Sciences
Cytotoxic T cell
Receptor
lcsh:Science
Immune Response
Antigen Presentation
Multidisciplinary
T Cells
Plant Anatomy
Hematology
Flow Cytometry
Body Fluids
Blood
Spectrophotometry
Antigens
Surface
Pollen
Female
Cytophotometry
Immunotherapy
Cellular Types
Anatomy
Research Article
Adult
Immune Cells
Antigen presentation
Immunology
Interleukin-3 Receptor alpha Subunit
Cytotoxic T cells
Biology
Research and Analysis Methods
Immunophenotyping
03 medical and health sciences
Young Adult
Immune system
medicine
Immune Tolerance
Humans
Lectins
C-Type
Blood Cells
lcsh:R
Biology and Life Sciences
Dendritic cell
Cell Biology
Dendritic Cells
Allergens
030104 developmental biology
Gene Expression Regulation
Desensitization
Immunologic
Immunoglobulin G
biology.protein
Clinical Immunology
lcsh:Q
Clinical Medicine
030215 immunology
Zdroj: PLoS ONE, Vol 11, Iss 2, p e0148838 (2016)
PLoS ONE
ISSN: 1932-6203
Popis: Allergen-specific immunotherapy (AIT) induces tolerance and shifts the Th2 response towards a regulatory T-cell profile. The underlying mechanisms are not fully understood, but dendritic cells (DC) play a vital role as key regulators of T-cell responses. DCs interact with allergens via Fc receptors (FcRs) and via certain C-type lectin receptors (CLRs), including CD209/DC-SIGN, CD206/MR and Dectin-2/CLEC6A. In this study, the effect of AIT on the frequencies as well as the FcR and CLR expression profiles of human DC subsets was assessed. PBMC was isolated from peripheral blood from seven allergic donors before and after 8 weeks and 1 year of subcutaneous AIT, as well as from six non-allergic individuals. Cells were stained with antibodies against DC subset-specific markers and a panel of FcRs and CLRs and analyzed by flow cytometry. After 1 year of AIT, the frequency of CD123+ DCs was increased and a larger proportion expressed FceRI. Furthermore, the expression of CD206 and Dectin-2 was reduced on CD141+ DCs after 1 year of treatment and CD206 as well as Dectin-1 was additionally down regulated in CD1c+ DCs. Interestingly, levels of DNGR1/CLEC9A on CD141+ DCs were increased by AIT, reaching levels similar to cells isolated from non-allergic controls. The modifications in phenotype and occurrence of specific DC subsets observed during AIT suggest an altered capacity of DC subsets to interact with allergens, which can be part of the mechanisms by which AIT induces allergen tolerance.
Databáze: OpenAIRE
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