Macrophage-derived exosomes attenuate fibrosis in airway epithelial cells through delivery of antifibrotic miR-142-3p
| Autor: | Franck Dequiedt, Maureen Cambier, Ingrid Struman, Edouard Louis, Olivier Nivelles, Julien Guiot, Fanny Gester, Monique Henket, Michel Malaise, Amandine Boeckx, Renaud Louis, Makon-Sébastien Njock |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Idiopathic pulmonary fibrosis Context (language use) Exosomes Interstitial Lung Disease Macrophage Biology 03 medical and health sciences 0302 clinical medicine Airway Epithelium Fibrosis Pulmonary fibrosis medicine Humans Aged 030304 developmental biology Aged 80 and over 0303 health sciences Lung business.industry Macrophages Interstitial lung disease Fibroblasts Middle Aged respiratory system medicine.disease Microvesicles respiratory tract diseases Interstitial Fibrosis MicroRNAs medicine.anatomical_structure Alveolar Epithelial Cells Case-Control Studies 030220 oncology & carcinogenesis Cancer research Sputum Female medicine.symptom business |
| Zdroj: | Thorax |
| ISSN: | 1468-3296 0040-6376 |
| DOI: | 10.1136/thoraxjnl-2019-214077 |
| Popis: | IntroductionIdiopathic pulmonary fibrosis (IPF) is a progressive fibrosing interstitial lung disease of unknown aetiology and cure. Recent studies have reported a dysregulation of exosomal microRNAs (miRs) in the IPF context. However, the impact of IPF-related exosomal miRs on the progression of pulmonary fibrosis is unknown.MethodsTwo independent cohorts were enrolled at the ambulatory care polyclinic of Liège University. Exosomes from sputum were obtained from 19 patients with IPF and 23 healthy subjects (HSs) (cohort 1), and the ones from plasma derived from 14 patients with IPF and 14 HSs (cohort 2). Exosomal miR expression was performed by quantitative reverse transcription–PCR. The functional role of exosomal miRs was assessed in vitro by transfecting miR mimics in human alveolar epithelial cells and lung fibroblasts.ResultsExosomal miR analysis showed that miR-142-3p was significantly upregulated in sputum and plasma of patients with IPF (8.06-fold, pDiscussionOur results suggest that macrophage-derived exosomes may fight against pulmonary fibrosis progression via the delivery of antifibrotic miR-142–3 p to alveolar epithelial cells and lung fibroblasts. |
| Databáze: | OpenAIRE |
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