Influence of bacteriocin-like substance, generation times, and genetic profiles of Listeria innocua on the isolation of Listeria monocytogenes
Autor: | Yayoi Shibusawa, Soichi Maruyama, Yasuji Katsube, Eiji Yokoyama, Takeshi Mikami |
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Rok vydání: | 2004 |
Předmět: |
DNA
Bacterial education Immunology Colony Count Microbial Biology medicine.disease_cause Microbiology Enrichment culture chemistry.chemical_compound Bacteriocin Listeria monocytogenes Bacteriocins medicine Immunology and Allergy Incubation General Veterinary General Medicine biology.organism_classification Random Amplified Polymorphic DNA Technique Infectious Diseases chemistry DNA profiling Listeria Food Microbiology Growth inhibition Bacteria |
Zdroj: | Comparative immunology, microbiology and infectious diseases. 28(3) |
ISSN: | 0147-9571 |
Popis: | Inhibition of isolation of Listeria monocytogenes by bacteriocin-like substance (BLS)-producing Listeria innocua after enrichment culture was investigated. When 26 L. monocytogenes strains were examined in combination with eight L. innocua strains using the spot on lawn method, 52/208 (25.0%) combinations showed the growth inhibition of L. monocytogenes. When two Listeria species were cultured simultaneously in selective enrichment broth, inhibition of isolation of L. monocytogenes was observed in 12/52 of the combinations at 24 h (23.1%), in 24/52 at 48 h (46.2%) and in 30/52 (57.7%) after 7 days of incubation. The randomly amplified polymorphic DNA profiles showed no interstrain similarities between either strains of the BLS-producing L. innocua or the BLS-sensitive L. monocytogenes strains. Therefore inhibition by BLS-producing L. innocua of isolation of L. monocytogenes after enrichment culture is unlikely to be dependent upon a particular genetic profile. |
Databáze: | OpenAIRE |
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