Induction of hair follicle dermal papilla cell properties in human induced pluripotent stem cell-derived multipotent LNGFR(+)THY-1(+) mesenchymal cells
| Autor: | Hideyuki Okano, Manabu Ohyama, Aki Tsukashima, Yo Mabuchi, Masayuki Amagai, Takashi Sasaki, Yumi Matsuzaki, Hironobu Okuno, Ophelia Veraitch |
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| Rok vydání: | 2017 |
| Předmět: |
Keratinocytes
0301 basic medicine Cellular differentiation Induced Pluripotent Stem Cells Nerve Tissue Proteins Tretinoin Receptors Nerve Growth Factor Mesenchymal Stem Cell Transplantation Article Cell Line Mice 03 medical and health sciences medicine Animals Humans Regeneration Induced pluripotent stem cell Cells Cultured Stem cell transplantation for articular cartilage repair Multidisciplinary integumentary system Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells medicine.disease Hair follicle Cell biology Endothelial stem cell 030104 developmental biology medicine.anatomical_structure Hair disease Multipotent Stem Cell Thy-1 Antigens Female Hair Follicle |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep42777 |
| Popis: | The dermal papilla (DP) is a specialised mesenchymal component of the hair follicle (HF) that plays key roles in HF morphogenesis and regeneration. Current technical difficulties in preparing trichogenic human DP cells could be overcome by the use of highly proliferative and plastic human induced pluripotent stem cells (hiPSCs). In this study, hiPSCs were differentiated into induced mesenchymal cells (iMCs) with a bone marrow stromal cell phenotype. A highly proliferative and plastic LNGFR(+)THY-1(+) subset of iMCs was subsequently programmed using retinoic acid and DP cell activating culture medium to acquire DP properties. The resultant cells (induced DP-substituting cells [iDPSCs]) exhibited up-regulated DP markers, interacted with human keratinocytes to up-regulate HF related genes, and when co-grafted with human keratinocytes in vivo gave rise to fibre structures with a hair cuticle-like coat resembling the hair shaft, as confirmed by scanning electron microscope analysis. Furthermore, iDPSCs responded to the clinically used hair growth reagent, minoxidil sulfate, to up-regulate DP genes, further supporting that they were capable of, at least in part, reproducing DP properties. Thus, LNGFR(+)THY-1(+) iMCs may provide material for HF bioengineering and drug screening for hair diseases. |
| Databáze: | OpenAIRE |
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