Engraftment of Prevascularized, Tissue Engineered Constructs in a Novel Rabbit Segmental Bone Defect Model
| Autor: | Alexandre Kaempfen, Sinan Güven, Dirk J. Schaefer, Ivan Martin, Claude Jaquiery, Arnaud Scherberich, Rene D. Largo, Atanas Todorov |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Callus formation medicine.medical_treatment Bone healing Mesenchymal Stem Cell Transplantation Transplantation Autologous Article Catalysis Bone resorption lcsh:Chemistry Inorganic Chemistry Tissue engineering medicine Animals Femur Bone Resorption Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Cells Cultured Spectroscopy Reduction (orthopedic surgery) Bone Transplantation Decellularization Tissue Engineering Tissue Scaffolds business.industry animal model Organic Chemistry Mesenchymal stem cell osteosynthesis vascularization General Medicine Computer Science Applications Surgery Disease Models Animal medicine.anatomical_structure lcsh:Biology (General) lcsh:QD1-999 decellularized bone Rabbits Bone marrow business Biomedical engineering |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 16, Iss 6, Pp 12616-12630 (2015) Volume 16 Issue 6 Pages 12616-12630 |
| ISSN: | 1422-0067 |
| Popis: | The gold standard treatment of large segmental bone defects is autologous bone transfer, which suffers from low availability and additional morbidity. Tissue engineered bone able to engraft orthotopically and a suitable animal model for pre-clinical testing are direly needed. This study aimed to evaluate engraftment of tissue-engineered bone with different prevascularization strategies in a novel segmental defect model in the rabbit humerus. Decellularized bone matrix (Tutobone) seeded with bone marrow mesenchymal stromal cells was used directly orthotopically or combined with a vessel and inserted immediately (1-step) or only after six weeks of subcutaneous "incubation" (2-step). After 12 weeks, histological and radiological assessment was performed. Variable callus formation was observed. No bone formation or remodeling of the graft through TRAP positive osteoclasts could be detected. Instead, a variable amount of necrotic tissue formed. Although necrotic area correlated significantly with amount of vessels and the 2-step strategy had significantly more vessels than the 1-step strategy, no significant reduction of necrotic area was found. In conclusion, the animal model developed here represents a highly challenging situation, for which a suitable engineered bone graft with better prevascularization, better resorbability and higher osteogenicity has yet to be developed. |
| Databáze: | OpenAIRE |
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