Phosphorylation of Native Porcine Olfactory Binding Proteins
| Autor: | Philippe Chemineau, Fanny Brimau, Chrystelle Le Danvic, Jean-Claude Michalski, Patricia Nagnan-Le Meillour |
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| Přispěvatelé: | ProdInra, Migration, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Union nationale des coopératives d’élevage et d’insémination animale (UNCEIA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2009 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Sus scrofa Olfaction [INFO] Computer Science [cs] Receptors Odorant Peptide Mapping Biochemistry DNA-binding protein Gas Chromatography-Mass Spectrometry 03 medical and health sciences Olfactory Mucosa BEMAD GEL DE POLYACRYLAMIDE Animals [INFO]Computer Science [cs] Secretion Phosphorylation Ecology Evolution Behavior and Systematics 030304 developmental biology WESTERN BLOT SPECTROMETRIE DE MASSE MALDI TOF 0303 health sciences biology Endoplasmic reticulum Binding protein 030302 biochemistry & molecular biology General Medicine [SDV] Life Sciences [q-bio] Secretory protein Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Odorant-binding protein biology.protein Lipocalin 1 |
| Zdroj: | Journal of Chemical Ecology Journal of Chemical Ecology, 2009, 35 (7), pp.752-760. ⟨10.1007/s10886-009-9663-z⟩ Journal of Chemical Ecology, Springer Verlag, 2009, 35 (7), pp.752-760. ⟨10.1007/s10886-009-9663-z⟩ |
| ISSN: | 1573-1561 0098-0331 |
| DOI: | 10.1007/s10886-009-9663-z |
| Popis: | Nagnan-Le Meillour: agent mis à disposition par l'unité INRA 1197.; International audience; The identification of various isoforms of olfactory binding proteins is of major importance to elucidate their involvement in detection of pheromones and other odors. Here, we report the characterization of the phosphorylation of OBP (odorant binding protein) and Von Ebner’s gland protein (VEG) from the pig, Sus scrofa. After labeling with specific antibodies raised against the three types of phosphorylation (Ser, Thr, Tyr), the phosphate-modified residues were mapped by using the beta-elimination followed by Michael addition of dithiothreitol (BEMAD) method. Eleven phosphorylation sites were localized in the pOBP sequence and nine sites in the VEG sequence. OBPs are secreted by Bowman’s gland cells in the extracellular mucus lining the nasal cavity. After tracking the secretion pathway in the rough endoplasmic reticulum of these cells, we hypothesize that these proteins may be phosphorylated by ectokinases that remain to be characterized. The existence of such a regulatory mechanism theoretically increases the number of OBP variants, and it suggests a more specific role for OBPs in odorant coding than the one of odorant solubilizer and transporter. |
| Databáze: | OpenAIRE |
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