Effect of calcitonin on cultured schwann cells
| Autor: | Feng-Yi Shen, Hani S. Matloub, John A. LoGiudice, Yuhui Yan, Michael A. Agresti, Ji-Geng Yan, Yu-Dong Gu, Robert J. Havlik, Lin-Ling Zhang, Jifeng Li |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Calcitonin Male medicine.medical_specialty Physiology chemistry.chemical_element Calcium Rats Sprague-Dawley 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Fluorescent light Physiology (medical) Internal medicine medicine Animals Propidium iodide Cells Cultured Calcium metabolism Bone Density Conservation Agents Regeneration (biology) Nerve injury Rats 030104 developmental biology Endocrinology chemistry Peripheral nerve injury Neurology (clinical) Schwann Cells medicine.symptom 030217 neurology & neurosurgery |
| Zdroj: | Musclenerve. 56(4) |
| ISSN: | 1097-4598 |
| Popis: | Introduction After nerve injury, calcium concentrations in intranerve fibers quickly increase. We have shown that functional recovery of injured nerves correlates with calcium absorption. A slight increase in calcium reduces the number of Schwann cells present. Calcitonin therapy greatly improves regeneration by accelerating calcium absorption. We examined the effect of adding calcitonin to higher concentration calcium media on cultured Schwann cells. Methods The cells, isolated from intact sciatic nerves, were cultured with normal or higher concentration calcium media with or without calcitonin. Schwann cells were incubated with anti-S-100, goat-anti-mouse, and propidium iodide and then viewed through fluorescent light and phase-contrast microscopy for observation and analysis. Results The cells in each calcitonin-containing medium showed many Schwann cells, however, the cells in the higher concentration calcium media showed fewer and more defective Schwann cells. Conclusion These results show that calcitonin protects against the harmful effects of excessive calcium encountered in peripheral nerve injury. Muscle Nerve 56: 768-772, 2017. |
| Databáze: | OpenAIRE |
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