SYT, a partner of SYT-SSX oncoprotein in synovial sarcomas, interacts with mSin3A, a component of histone deacetylase complex
| Autor: | Tatsuo Ito, Kenji Shimizu, Sachio Ito, Yoshimi Jitsumori, Yuki Morimoto, Mamoru Ouchida, Akira Kawai, Toshifumi Ozaki, Hajime Inoue |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Oncogene Proteins
Fusion Two-hybrid screening Soft Tissue Neoplasms Biology Gene Expression Regulation Enzymologic Histone Deacetylases Pathology and Forensic Medicine Cell Line Fusion gene Sarcoma Synovial Proto-Oncogene Proteins Two-Hybrid System Techniques medicine Humans Luciferase Molecular Biology Gene chemistry.chemical_classification Proteins Cell Biology medicine.disease Molecular biology Synovial sarcoma Amino acid Repressor Proteins Sin3 Histone Deacetylase and Corepressor Complex chemistry Histone deacetylase complex Trans-Activators Histone deacetylase Transcription Factors |
| Zdroj: | Laboratory investigation; a journal of technical methods and pathology. 84(11) |
| ISSN: | 0023-6837 |
| Popis: | Synovial sarcomas are soft-tissue tumors predominantly affecting children and young adults. They are molecular-genetically characterized by the SYT-SSX fusion gene generated from chromosomal translocation t(X; 18) (p11.2; q11.2). When we screened new gene products that interact with SYT or SSX proteins by yeast two-hybrid assay, we found that mSin3A, a component of the histone deacetylase complex, interacts with SYT but not with SSX. These results were confirmed by mammalian two-hybrid and pull-down assays. Analyses with sequential truncated proteins revealed a main mSin3A-interaction region on the SYT amino-terminal 93 amino acids, and another one on the region between 187th amino acid and break point. In luciferase assay, mSin3A repressed the transcriptional activity of reporter promoter mediated by SYT and hBRM/BRG1. Our results suggest that the histone deacetylase complex containing mSin3A may regulate the transcriptional activation mediated by SYT. |
| Databáze: | OpenAIRE |
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