Immunological marker analysis of mitogen-induced proliferating lymphocytes using BrdU incorporation or screening of metaphases. Staphylococcal protein A is a potent mitogen for CD4+ lymphocytes
| Autor: | Herbert Hooijkaas, J. H. F. M. Wijdenes‐De Brfsser, H. J. Adriaansen, C. Osman, J. J. M. Dongen, C. M. J. M. Kappetijn‐Van Tilborg |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Immunology chemical and pharmacologic phenomena Lymphocyte Activation Peripheral blood mononuclear cell Immunophenotyping Concanavalin A Humans Phytohemagglutinins Staphylococcal Protein A Metaphase biology Pokeweed mitogen General Medicine T lymphocyte Molecular biology In vitro Bromodeoxyuridine Pokeweed Mitogens Cell culture biology.protein Mitogens Protein A CD8 Biomarkers Cell Division |
| Zdroj: | Scandinavian journal of immunology. 32(6) |
| ISSN: | 0300-9475 |
| Popis: | The proliferative effects of the mitogens phylohaemagglutinin (PHA). concanavalin A (Con A), pokeweed mitogen (PWM). and staphylococcal protein A (SpA) were investigated using two different methods which enable immunological marker analysis of proliferating cells: either surface marker labelling followed by Brd U incorporation or screening of metaphases after surface marker labelling. Therefore peripheral blood mononuclear cells from six healthy volunteers were stimulated with these four mitogens. Both PHA and Con A gave rise to more CD8+ than CD4+ proliferating cells. PHA, but not Con A, induced B-cell proliferation as well. PWM mainly caused T-cell proliferation. SpA also appeared to be a potent T-cell mitogen in addition to its capacity to induce B-cell proliferation. However, in contrast to the other mitogens SpA predominantly stimulated CD4+ cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text