| Autor: |
Tahir, Noor Mohammadi, Cunkuan, Shen, Yuncheng, Li, Mahmoud Gamaleldin, Zayda, Jun, Sato, Yoshimitsu, Masuda, Ken-Ichi, Honjoh, Takahisa, Miyamoto |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
International Journal of Food Microbiology. 380:109886 |
| ISSN: |
0168-1605 |
| DOI: |
10.1016/j.ijfoodmicro.2022.109886 |
| Popis: |
Clostridium perfringens is a major cause of foodborne disease in developed countries. The aim of this study was to isolate and characterize phages specific to C. perfringens to evaluate the most efficient phage cocktail for the biocontrol of C. perfringens, both in vitro and in curry roux. In this study, four phages were isolated from chicken meat and were morphologically and genetically characterized along with two phages previously isolated in our laboratory that display different host lysis spectra. Phage cocktail CP11, consisting of phages CPQ3, 7, 8, and 10, showed the broadest host range. Electron micrograph images suggested that all four phages belong to the Podoviridae family, and none of them carry any antibiotic resistance or toxin genes. Notably, the phages were stable at various pH values and in curry roux. Cocktails consisting of six, five, and four phages at the same concentrations were examined to determine the most effective phage cocktail. Phage cocktail PC11 significantly decreased the viable count of C. perfringens to a value less than the lower detection limit up to 48 h at both 8 and 37 °C in broth and at 24 °C in the curry roux. These results suggest that phage cocktail PC11 is a promising natural biocontrol agent against C. perfringens in vitro and in curry roux. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect