Microbiological comparative study of isolates of Edwardsiella tarda isolated in different countries from fish and humans
| Autor: | C Nucci, M.A Ribeiro, S da Silva Corrêa, Gerson Nakazato, A.F. Pestana de Castro, W D Da Silveira, Silvia Yumi Bando |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Bacterial Toxins medicine.disease_cause Polymerase Chain Reaction Microbiology Bacterial Adhesion law.invention Fish Diseases chemistry.chemical_compound law medicine Animals Humans Edwardsiella tarda Enteroinvasive Escherichia coli Polymerase chain reaction Intimin General Veterinary biology Enterobacteriaceae Infections Fishes Shiga toxin Hemolysin General Medicine biology.organism_classification Random Amplified Polymorphic DNA Technique RAPD Microscopy Electron chemistry Fimbriae Bacterial biology.protein Aerobactin Female HeLa Cells |
| Zdroj: | Veterinary Microbiology. 89:29-39 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/s0378-1135(02)00151-7 |
| Popis: | It is difficult to use tissue culture assays to investigate adherence and other properties of Edwardsiella tarda because the organism is invasive and produces a potent hemolysin. We therefore relied on polymerase chain reaction (PCR) to determine the occurrence of genes for enterotoxins (LT-I, EAST-1), Shiga toxin (Stx-1, Stx-2), cytotoxic necrotizing factors (CNF-1, CNF-2), aerobactin, invasion plasmid of enteroinvasive Escherichia coli, EPEC adherence factor (EAF), intimin (Eae), enterohemolysin (EntHly) and hemolysin (Hly) in 53 isolates of E. tarda from humans and fish from several countries. All isolates were negative for all genes investigated by PCR. Adhesion to and invasion of HeLa cells were determined by using the unusually short incubation time of 1h or 30 min. All isolates adhered and invaded in these tests. Finally, a random amplified polymorphic DNA (RAPD) test distinguished, with a few exceptions, isolates of human and fish origin. |
| Databáze: | OpenAIRE |
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