Environmental pollution reducing strategy for scouring of undegummed sisal fibers using xylanase and pectinase enzymes
| Autor: | Libin Mathew Varghese, A K Patra, Ritu Mahajan, Bindu Battan, Avtar Singh, Rishi Pal Mandhan |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Molar concentration Bioengineering Environmental pollution 01 natural sciences Buffer (optical fiber) Bacterial Proteins 010608 biotechnology Pectinase SISAL computer.programming_language Bacillus pumilus chemistry.chemical_classification Endo-1 4-beta Xylanases 010405 organic chemistry General Medicine Hydrogen-Ion Concentration Pulp and paper industry 0104 chemical sciences Enzyme Polygalacturonase chemistry Xylanase Pectins Industrial and production engineering computer Biotechnology |
| Zdroj: | Bioprocess and biosystems engineering. 44(3) |
| ISSN: | 1615-7605 |
| Popis: | This study was undertaken to investigate the potential of bioscouring in the processing of undegummed sisal fibers, using xylano-pectinolytic enzymes. Optimum bioscouring was obtained at pH 8.5 and 50 mM buffer molarity, using xylanase (10 IU) and pectinase (8 IU), with a material to liquor proportion of 1:25 (g:ml), EDTA (2 mM) and Tween 80 (0.5%), at 50 °C temperature with agitation rate of 55 rpm and treatment period of 60 min. Enzymatic treatment of sisal fibers enhanced the brightness and whiteness by 11.52 and 6.83%, respectively, and reduced the yellowness by 7.14% in comparison to control. The use of xylanase and pectinase enzymes completely replaced the chemical scouring method for removing non-cellulosic impurities. Thus, enzymatic scouring is energy saving and ecofriendly, since it completely eliminated the use of toxic chemicals used in alkaline scouring. An increase of 23.75% and 11.58% in brightness and whiteness of enzymatically scoured cum bleached fibers, as compared to chemically scoured cum bleached fibers was finally obtained, along with reduction in yellowness by 27.99%. This is the first report demonstrating environmentally sustainable enzymatic approach for scouring of undegummed sisal fibers, using enzymes, simultaneously produced from a bacterial isolate. |
| Databáze: | OpenAIRE |
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