Loss of the Desmosomal Component Perp Impairs Wound HealingIn Vivo
| Autor: | Bichchau Nguyen, Suzanne B.R. Jacobs, Navneeta Pathak, Rebecca A. Ihrie, Veronica G. Beaudry, Laura D. Attardi, Eunice J. Park |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Article Subject Dermatology 03 medical and health sciences 0302 clinical medicine In vivo Conditional gene knockout lcsh:Dermatology medicine Keratinocyte migration Cell adhesion 030304 developmental biology 0303 health sciences integumentary system business.industry Adhesion lcsh:RL1-803 Surgery Cell biology medicine.anatomical_structure 030220 oncology & carcinogenesis Wound closure business Keratinocyte Wound healing Research Article |
| Zdroj: | Dermatology Research and Practice, Vol 2010 (2010) Dermatology Research and Practice |
| ISSN: | 1687-6113 1687-6105 |
| DOI: | 10.1155/2010/759731 |
| Popis: | Epithelial wound closure is a complex biological process that relies on the concerted action of activated keratinocytes and dermal fibroblasts to resurface and close the exposed wound. Modulation of cell-cell adhesion junctions is thought to facilitate cellular proliferation and migration of keratinocytes across the wound. In particular, desmosomes, adhesion complexes critical for maintaining epithelial integrity, are downregulated at the wound edge. It is unclear, however, how compromised desmosomal adhesion would affect wound reepithelialization, given the need for a delicate balance between downmodulating adhesive strength to permit changes in cellular morphology and maintaining adhesion to allow coordinated migration of keratinocyte sheets. Here, we explore the contribution of desmosomal adhesion to wound healing using mice deficient for the desmosomal component Perp. We find thatPerpconditional knockout mice display delayed wound healing relative to controls. Furthermore, we determine that while loss of Perp compromises cell-cell adhesion, it does not impair keratinocyte proliferation and actually enhances keratinocyte migration inin vitroassays. Thus, Perp's role in promoting cell adhesion is essential for wound closure. Together, these studies suggest a role for desmosomal adhesion in efficient wound healing. |
| Databáze: | OpenAIRE |
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