Programmable Peptide-Cross-Linked Nucleic Acid Nanocapsules as a Modular Platform for Enzyme Specific Cargo Release
| Autor: | Binglin Sui, Joshua J. Santiana, Jessica L. Rouge, Nicole Gomez |
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| Rok vydání: | 2017 |
| Předmět: |
Azides
Proteases medicine.medical_treatment Biomedical Engineering Metal Nanoparticles Pharmaceutical Science Bioengineering Peptide 02 engineering and technology 010402 general chemistry 01 natural sciences Nanocapsules Enflurane Nucleic Acids medicine Humans Sulfhydryl Compounds Pharmacology chemistry.chemical_classification Drug Carriers Protease Chemistry Organic Chemistry Biological Transport 021001 nanoscience & nanotechnology 0104 chemical sciences Drug Liberation Matrix Metalloproteinase 9 Drug delivery Nucleic acid Biophysics Gold Peptides 0210 nano-technology Drug carrier Biosensor HeLa Cells Biotechnology |
| Zdroj: | Bioconjugate Chemistry. 28:2910-2914 |
| ISSN: | 1520-4812 1043-1802 |
| Popis: | Herein we describe a modular assembly strategy for photo-cross-linking peptides into nucleic acid functionalized nanocapsules. The peptides embedded within the nanocapsules form discrete nanoscale populations capable of gating the release of molecular and nanoscale cargo using enzyme-substrate recognition as a triggered release mechanism. Using photocatalyzed thiol-yne chemistry, different peptide cross-linkers were effectively incorporated into the nanocapsules and screened against different proteases to test for degradation specificity both in vitro and in cell culture. By using a combination of fluorescence assays, confocal and TEM microscopy, the particles were shown to be highly specific for their enzyme targets, even between enzymes of similar protease classes. The rapid and modular nature of the assembly strategy has the potential to be applied to both intracellular and extracellular biosensing and drug delivery applications. |
| Databáze: | OpenAIRE |
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