The effects of herkinorin, the first mu-selective ligand from a salvinorin A-derived scaffold, in a neuroendocrine biomarker assay in nonhuman primates
| Autor: | Mary Jeanne Kreek, Eduardo R. Butelman, Szymon Rus, Angela K.H. Wolf, Thomas E. Prisinzano, Denise S. Simpson |
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| Rok vydání: | 2008 |
| Předmět: |
Agonist
Male medicine.medical_specialty medicine.drug_class Receptors Opioid mu Pharmacology Biology Loperamide Naltrexone Article Diterpenes Clerodane chemistry.chemical_compound In vivo Internal medicine medicine Animals Furans Nalmefene Dose-Response Relationship Drug Ligand (biochemistry) Macaca mulatta Prolactin Salvinorin A Endocrinology chemistry Pyrones Molecular Medicine Female Biomarkers medicine.drug Herkinorin |
| Zdroj: | The Journal of pharmacology and experimental therapeutics. 327(1) |
| ISSN: | 1521-0103 |
| Popis: | Herkinorin is the first mu-opioid receptor-selective ligand from the salvinorin A diterpenoid scaffold. Herkinorin has relative mukappadelta binding selectivity, and it can act as an agonist at both mu- and kappa-receptors, in vitro. These studies were the first in vivo evaluation of the effects of herkinorin in nonhuman primates, using prolactin release, a neuroendocrine biomarker assay that is responsive to both mu- and kappa-agonists, as well as to compounds with limited ability to cross the blood-brain barrier. In cumulative dosing studies (0.01-0.32 mg/kg i.v.), herkinorin produced only small effects in gonadally intact males (n = 4), but a more robust effect in females (n = 4). Time course studies with herkinorin (0.32 mg/kg) confirmed this greater effectiveness in females and revealed a fast onset after i.v. administration (e.g., by 5-15 min). Antagonism experiments with different doses of nalmefene (0.01 and 0.1 mg/kg) caused dose-dependent and complete prevention of the effect of herkinorin in females. This is consistent with a principal mu-agonist effect of herkinorin, with likely partial contribution by kappa-agonist effects. The peripherally selective antagonist quaternary naltrexone (1 mg/kg s.c.) caused approximately 70% reduction in the peak effect of herkinorin (0.32 mg/kg) in females, indicating that this effect of herkinorin is prominently mediated outside the blood-brain barrier. |
| Databáze: | OpenAIRE |
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