Apoptosis differentially regulates mesenteric and subcutaneous lymph node immune responses to Trypanosoma cruzi
| Autor: | George A. DosReis, Elisabeth M. Silva, Marcela F. Lopes, Juliana de Meis, Landi V. C. Guillermo, Lidia M. S. Ferreira |
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| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_treatment T-Lymphocytes Trypanosoma cruzi Immunology Apoptosis Enzyme-Linked Immunosorbent Assay Parasitemia Lymphocyte Activation digestive system Mice Immune system parasitic diseases medicine Immunology and Allergy Animals Chagas Disease Mesentery Enzyme Inhibitors Lymph node Caspase Skin Mice Inbred BALB C biology digestive oral and skin physiology medicine.disease biology.organism_classification Flow Cytometry body regions medicine.anatomical_structure Cytokine Caspases biology.protein Cytokines Lymph Lymph Nodes Atrophy |
| Zdroj: | European journal of immunology. 38(1) |
| ISSN: | 0014-2980 |
| Popis: | Infection with Trypanosoma cruzi causes expansion of subcutaneous (SLN) and atrophy of mesenteric (MLN) lymph nodes. Here we show that excision of MLN increased parasitemia in T. cruzi-infected mice. We then studied how apoptosis of MLN cells affects immune responses to infection. T cell apoptosis increased in the MLN compared to SLN in T. cruzi-infected mice. Absolute numbers of naive T cells decreased, and activated T cells failed to accumulate in MLN during infection. In addition, activated T cells from MLN produced less IL-2, IFN-gamma, IL-4, and IL-10 than T cells from SLN. Treatment with IL-4 or with caspase-9 inhibitor increased the recovery of viable T cells in vitro. Treatment with caspase-9 inhibitor also increased the production of cytokines by MLN T cells from infected mice. Moreover, injection of a pan caspase inhibitor prevented MLN atrophy during T. cruzi infection. Caspase-9, but not caspase-8, inhibitor also reduced MLN atrophy and increased the recovery of naive and activated T cells from MLN. These findings indicate that caspase-mediated apoptosis and defective cytokine production are implicated in MLN atrophy and affect immune responses to T. cruzi infection. |
| Databáze: | OpenAIRE |
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