Dimethyl sulfoxide cataract: a model for optical anisotropy fluctuations
| Autor: | A. C. Churchill, J. S. Zigler, W G Robison, Frederick A. Bettelheim |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Birefringence
Dimethyl sulfoxide business.industry Biomedical Engineering Mole fraction Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Biomaterials chemistry.chemical_compound Optics chemistry Fiber cell Biophysics medicine Fiber Turbidity Swelling medicine.symptom Anisotropy business |
| Zdroj: | Journal of biomedical optics. 1(3) |
| ISSN: | 1083-3668 |
| Popis: | Rat lenses incubated in dimethyl sulfoxide (DMSO)‐water binary mixtures of different compositions became turbid. A slight haziness developed up to 0.062 mole fraction of DMSO; at higher concentrations dense turbidity developed. Microscopic examination of the incubated rat lenses showed damage restricted to the epithelium and outer cortex at low DMSO concentrations; maximum damage occurred, in terms of fiber cell swelling, extracellular fluid (lake) formation and disintegration of epithelium, at 0.25 mole ratio of DMSO. Scanning electron microscopic observation showed that at high DMSO concentrations, especially at 0.25 mole fraction, the contents of the fiber cells were greatly damaged. Apparently a large part of the crystallins had coalesced around the cytoskeletal bodies. Polarized light-scattering intensity measurements and their analyses indicated that when dense opacities developed at high DMSO concentrations, the major contribution to the turbidity came from the optical anisotropy fluctuations. The change in the organization of the components within the fiber cells disturbs the balance between intrinsic birefringence and form birefringence necessary for transparency. Thus, the DMSO-caused opacification can be described as a good model for the involvement of optical anisotropy fluctuations in cataractogenesis. © 1996 Society of Photo-Optical Instrumentation Engineers. |
| Databáze: | OpenAIRE |
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