Cutting Edge: A Dual Role for Type I IFNs during Polyinosinic-Polycytidylic Acid-Induced NK Cell Activation
| Autor: | Isabelle Bouvier, James P. Di Santo, Matthew L. Albert, Jacques Deguine, Hélène Beuneu, Philippe Bousso |
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| Přispěvatelé: | Dynamiques des Réponses Immunes, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Immunobiologie des Cellules Dendritiques, Immunité Innée, This work was supported by the Institut Pasteur, INSERM, the European Research Council, Mairie de Paris, and a Marie-Curie Excellence grant. H.B. is supported by the Fondation pour la Recherche Me´dicale. M.L.A. is supported by the Ligue contre le Cancer and a European Research Council award., Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Rok vydání: | 2011 |
| Předmět: |
Adoptive cell transfer
Interferon Inducers MESH: Interferon Inducers/immunology Immunology MESH: Killer Cells Natural/immunology Priming (immunology) MESH: Flow Cytometry Cell Separation Biology Lymphocyte Activation MESH: Cell Separation MESH: Interferon Type I/immunology Mice 03 medical and health sciences chemistry.chemical_compound Interleukin 21 0302 clinical medicine MESH: Mice Inbred C57BL MESH: Poly I-C/immunology Animals Immunology and Allergy Cytotoxic T cell MESH: Animals MESH: Mice 030304 developmental biology MESH: Signal Transduction/immunology Interleukin-15 0303 health sciences Lymphokine-activated killer cell Janus kinase 3 MESH: Lymphocyte Activation/immunology MESH: Interleukin-15/immunology Flow Cytometry Adoptive Transfer Cell biology Killer Cells Natural Mice Inbred C57BL MESH: Adoptive Transfer Poly I-C chemistry Polyinosinic:polycytidylic acid Interferon Type I Interleukin 12 [SDV.IMM]Life Sciences [q-bio]/Immunology Signal Transduction 030215 immunology |
| Zdroj: | Journal of Immunology Journal of Immunology, 2011, 187 (5), pp.2084-8. ⟨10.4049/jimmunol.1004210⟩ Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2011, 187 (5), pp.2084-8. ⟨10.4049/jimmunol.1004210⟩ |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.1004210 |
| Popis: | NK cells are cytotoxic lymphocytes that are most efficient at fulfilling their functions after a phase of priming provided by cytokines and/or accessory cells. Although type I IFNs are known to be important in this process, it remains unclear whether they act directly on NK cells or indirectly on accessory cells. We used adoptive transfer experiments and mixed bone marrow chimeras to dissect the requirement for type I IFN signaling in response to the dsRNA analog polyinosinic-polycytidylic acid. We demonstrate that optimal NK cell priming requires type I IFNs to signal on both NK cells and accessory cells. In the absence of IL-15, the residual NK cell activation was strictly dependent on cell-intrinsic IFNAR signaling in NK cells. Our results suggest that type I IFNs produced following viral infection simultaneously target accessory cells for IL-15 transpresentation and NK cells themselves and that these two pathways cooperate for NK cell priming. |
| Databáze: | OpenAIRE |
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