Towards Autonomous Process Control—Digital Twin for HIV-Gag VLP Production in HEK293 Cells Using a Dynamic Metabolic Model
| Autor: | Heribert Helgers, Alina Hengelbrock, Jamila Franca Rosengarten, Jörn Stitz, Axel Schmidt, Jochen Strube |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
digital twin (DT) -- advanced process control (APC) -- quality-by-design (QbD) -- process analytical technology (PAT) -- real-time-release testing (RTRT) -- human embryonic kidney 293 cells (HEK293) -- human immunodeficiency virus (HIV) -- virus-like particles (VLPs)
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften Biologie digital twin (DT) human embryonic kidney 293 cells (HEK293) Process Chemistry and Technology advanced process control (APC) quality-by-design (QbD) process analytical technology (PAT) real-time-release testing (RTRT) human immunodeficiency virus (HIV) virus-like particles (VLPs) article Bioengineering Virus-like particles Digitaler Zwilling ddc:570 Chemical Engineering (miscellaneous) ddc:500 ddc:620 |
| Zdroj: | Processes; Volume 10; Issue 10; Pages: 2015 Processes : open access journal 10 (2022), Nr. 10 Processes : open access journal |
| ISSN: | 2227-9717 |
| DOI: | 10.3390/pr10102015 |
| Popis: | Despite intensive research over the last three decades, it has not yet been possible to bring an effective vaccine against human immunodeficiency virus (HIV) and the resulting acquired immunodeficiency syndrome (AIDS) to market. Virus-like particles (VLP) are a promising approach for efficient and effective vaccination and could play an important role in the fight against HIV. For example, HEK293 (human embryo kidney) cells can be used to produce virus-like particles. In this context, given the quality-by-design (QbD) concept for manufacturing, a digital twin is of great importance for the production of HIV-Gag-formed VLPs. In this work, a dynamic metabolic model for the production of HIV-Gag VLPs was developed and validated. The model can represent the VLP production as well as the consumption or formation of all important substrates and metabolites. Thus, in combination with already described process analytical technology (PAT) methods, the final step towards the implementation of a digital twin for process development and design, as well as process automation, was completed. |
| Databáze: | OpenAIRE |
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