Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender

Autor: Burcu Üstüner, Cafer Ceniz, Elif Gokce, Selim Alcay, Hakan Sagirkaya, Zekariya Nur, Zulfiye Gul, Muhammed Duman, M. Berk Toker, M. Kemal Soylu, Fusun Ak Sonat, Aydın Uslu
Přispěvatelé: Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı., Uludağ Üniversitesi/Veteriner Fakültesi/Fizyoloji Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı., Uludağ Üniversitesi/Veteriner Fakültesi/Su Hayvanları Hastalıkları Anabilim Dalı., Üstüner, Burcu, Alcay, Selim, Toker, M. Berk, Nur, Zekariya, Gökce, Elif, Sonat, Füsun Ak, Gül, Zülfiye, Duman, Muhammed, Cenize, Cafer, Uslu, Aydın, Saığrkaya, Hakan, Soylu, Mustafa Kemal, AAH-2635-2021, T-1697-2019, AAH-8821-2021, AAF-9939-2020, AAG-7238-2021
Rok vydání: 2015
Předmět:
Veterinary sciences
Glycerol
Male
Physiology
Unfrozen
Agriculture
dairy & animal science
Lecithin
Cryopreservation
law.invention
In-vitro
0302 clinical medicine
Endocrinology
Cryoprotective Agents
Food Animals
law
Capacitation
Malondialdehyde
Cryoprotective agent
Sperm motility
Seminal plasma
030219 obstetrics & reproductive medicine
Extender
Malonaldehyde
Agriculture
04 agricultural and veterinary sciences
General Medicine
Plant lectin
Semen cryopreservation
Spermatozoa
Egg Yolk
Semen
Semen Extenders
Rainbow trout
Veterinary
Oncorhynchus mykiss
Soybean Proteins
Soybean protein
Plant Lectins
Acrosome
food.ingredient
Reproductive biology
Biology
Andrology
03 medical and health sciences
food
Yolk
Sperm preservation
Animals
Hypoosmotic swelling test
Sheep
Animal
Cell Membrane
0402 animal and dairy science
Soybean lectin
040201 dairy & animal science
Sperm
Ram semen
Fertility
Parameters
Animal Science and Zoology
Cold-shock
Semen Preservation
Zdroj: Animal reproduction science. 164
ISSN: 1873-2232
Popis: The aim of the current study was to evaluate the effects of different concentrations of rainbow trout seminal plasma (RTSP) (0.1%, 1% and 10%) in extenders containing either egg yolk or lecithin for use in Awassi ram semen cryopreservation. Pooled sperm were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in egg yolk or lecithin extender containing no RTSP, 0.1%, 1% or 10% RTSP (v/v). Semen samples were assessed for sperm motility, plasma membrane integrity [hypoosmotic swelling test (HOST) and Hoechst 33258] and defective acrosomes [FITC-conjugated Pisum sativum agglutinin (PSA-FITC)] at the following five time points: after dilution with extender A; after equilibration; and post-thaw at 0 h, 3 h and 5 h. Malondialdehyde (MDA) was examined only after thawing. Freezing and thawing procedures (dilution, equilibration and post-thaw incubation at Oh, 3 h and 5 h) negatively affected the motility (P
Databáze: OpenAIRE
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