Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp
| Autor: | Skorn Mongkolsuk, Herbert P. Schweizer, Mayuree Fuangthong, Paiboon Vattanaviboon, Sarinya Buranajitpakorn, Thichakorn Jittawuttipoka |
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| Rok vydání: | 2009 |
| Předmět: |
Genetics
Transposable element DNA Bacterial Genetic Vectors Molecular Sequence Data Bacterial genome size Sequence Analysis DNA Biology biology.organism_classification Xanthomonas campestris Microbiology Insert (molecular biology) Article Bacterial genetics Mutagenesis Insertional Xanthomonas oryzae Xanthomonas Recombinase DNA Transposable Elements Genetic Engineering Molecular Biology |
| Zdroj: | FEMS microbiology letters. 298(1) |
| ISSN: | 1574-6968 |
| Popis: | Transposon mini-Tn7 vectors insert into the chromosome of several Gram-negative bacteria in a site-specific manner. Here, we showed the application of mini-Tn7 as single copy site-specific integration vector system for Xanthomonas campestris pv. campestris. The transposition of the mini-Tn7 into the bacterial genome was detected at a Tn7 attachment (attTn7) site located downstream of glmS1. Furthermore, using a newly constructed vector pBBR1FLP2 containing the flipase (FLP) recombinase for site-specific excision of the sequence between the FLP recombinase target (FRT) sites, and a sacB counter selection marker, an unmarked mini-Tn7 insertion mutant was created. Mini-Tn7 insertion did not affect bacterial virulence on the tested plant. The mini-Tn7 and FLP-FRT systems also work well in Xanthomonas oryzae pv. oryzae. |
| Databáze: | OpenAIRE |
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