Sphingobacterium populi sp. nov., isolated from bark of Populus × euramericana
| Autor: | Min-wei Guo, Wang Laifa, Yan Li, Liang Wx, Song Lm |
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| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0301 basic medicine China Biology Microbiology 03 medical and health sciences RNA Ribosomal 16S Populus euramericana Botany Sphingobacterium Phospholipids Phylogeny Ecology Evolution Behavior and Systematics Base Composition Sphingobacterium populi Fatty Acids Vitamin K 2 Sequence Analysis DNA General Medicine Bacterial Typing Techniques Populus 030104 developmental biology visual_art Plant Bark visual_art.visual_art_medium Bark |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 66:3456-3462 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | A Gram-stain-negative, aerobic, non-motile bacterial strain, 7Y-4T, was isolated from bark tissue of Populus × euramericana. The isolate was able to grow between 10 and 37 °C, with optimal growth occurring at 28-30 °C. Strain 7Y-4T was positive for oxidase and catalase activities, but did not reduce nitrite from nitrate. Positive reactions were observed for the activities of β-galactosidase, urease and β-glucosidase, but negative reactions for the activities of gelatinase and the production of indole, acetoin and H2S. Citrate was not utilized. The major fatty acids of strain 7Y-4T are iso-C15 : 0 (28.6 %), C16 : 1ω7c/C16 : 1ω6c (31.8 %) and iso-C17 : 0 3-OH (23.3 %).The major polar lipids of the novel isolate include phosphatidylethanolamine, three unknown phospholipids (PL1-3) and six unknown lipids (L1-6), and the predominant menaquinone is MK-7. The DNA G+C content is 41.7 mol%. Analysis of 16S rRNA gene sequences revealed that the novel isolate shared the greatest sequence similarity with Sphingobacterium hotanense XH4T (93.50 %). On the basis of phenotypic and genotypic characteristics, strain 7Y-4T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium populi is proposed. The type strain is 7Y-4T (=CFCC 11742T=KCTC 42247T). |
| Databáze: | OpenAIRE |
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