Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models
| Autor: | Nina Petrović, Sema Misir, Kalbiye Konanç, Marija Đorđić Crnogorac, Ivana Z. Matić, Sercan Ergun, Hurija Džudžević-Čančar, Tatjana Stanojković, Ana Damjanović, Yuksel Aliyazicioglu |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
miR128/193a-5p/335 Clinical Biochemistry Biomedical Engineering Bioengineering Matrix-metalloproteinase 3D HeLa spheroids Flow cytometry 3D HeLa spher HeLa 03 medical and health sciences 0302 clinical medicine medicine Hypericum perforatum Cytotoxic T cell Cytotoxicity biology medicine.diagnostic_test Cytotoxic activity Chemistry Cell Biology Cell cycle biology.organism_classification Molecular biology 030104 developmental biology Apoptosis 030220 oncology & carcinogenesis Cancer cell Vascular endothelial growth factor Biotechnology |
| Zdroj: | Cytotechnology |
| Popis: | Six extracts were obtained from plant speciesHypericum perforatumL., collected at Samsun in Turkey. The aim of this study was to examine the mechanisms of the anticancer activity of these extracts. Methanol, ethyl-acetate and hexane were used as a solvents for extraction from both branch-body part of the plant (extracts 1, 2 and 3) and from plant flowers (extracts 4, 5 and 6). The cytotoxic effects of the extracts were determined against 2D and 3D cancer cell models. Cell cycle changes of treated HeLa cells were analyzed by flow cytometry. Measurements of gene and microRNA expression levels in treated HeLa cells weredone by quantitative real time PCR. Five examined extracts (2–6) exerted selective concentration-dependent cytotoxic effects on HeLa, K562, and A549 cancer cells, while the extract 1 exhibited very weak cytotoxicity. The extract 6 showed the highest intensity of cytotoxic activity. All tested extracts (2–6) demonstrated the ability to induce apoptosis in HeLa cells through activation of caspase-3. These extracts remarkably decreased gene expression levels ofMMP2,MMP9,TIMP3, andVEGFAin HeLa cells. Flower extracts might have stronger effects on miR128/193a-5p/335 level changes than branch-body extracts.Hypericum perforatumextracts exerted weaker cytotoxic effects on 3D HeLa spheroids when compared with their effects on 2D monolayer HeLa cells. Taken together, results of our research may suggest the promising anticancer properties of theHypericum perforatumextracts. |
| Databáze: | OpenAIRE |
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