Comparison of the bone protective effects of an isoflavone-rich diet with dietary and subcutaneous administrations of genistein in ovariectomized rats
| Autor: | B. Schleipen, U. Laudenbach, C. Offermanns, Patrick Diel, M. Velders, T. Hertrampf |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Injections Subcutaneous Ovariectomy Osteocalcin Genistein Phytoestrogens Biology Toxicology Collagen Type I Bone resorption Biochanin A chemistry.chemical_compound Bone Density Osteogenesis Internal medicine medicine Animals Bone Resorption Rats Wistar Pyridinoline Bone Density Conservation Agents Estradiol Tibia Uterus Daidzein Organ Size General Medicine Isoflavones Diet Rats Disease Models Animal Endocrinology chemistry Ovariectomized rat biology.protein Osteoporosis Female Osteopontin Peptides |
| Zdroj: | Toxicology Letters. 184:198-203 |
| ISSN: | 0378-4274 |
| DOI: | 10.1016/j.toxlet.2008.11.006 |
| Popis: | Administration of the isoflavone genistein (GEN) has been described to result in bone protection but also to induce uterotrophic responses. To compare bone protective effects of GEN with an isoflavone-rich diet (IRD) and to further elucidate molecular mechanisms involved in bone-protection, ovariectomized rats (OVX) received either a diet low in isoflavone content (IDD) enriched with GEN (42 mg kg −1 b.wt d −1 ) (GEN d ), an IRD (14 mg kg −1 b.wt d −1 GEN, 14 mg kg −1 b.wt d −1 daidzein) or were treated subcutaneously (s.c.) with GEN (10 mg kg −1 b.wt d −1 ) (GEN sc ) for 12 weeks. Intact (SHAM), vehicle treated OVX animals and those substituted with 17β-estradiol (2 μg kg −1 b.wt d −1 ) (E 2 ), served as controls. OVX-induced bone loss could be antagonized in E 2 , GEN sc , GEN d and IRD groups. Uterine wet weight (UWW) was only stimulated in E 2 and GEN sc animals. Serum biomarkers of bone-formation (osteocalcin, osteopontin) and bone-resorption (telopeptides of collagen type I, pyridinoline cross-links) were elevated in OVX compared to SHAM and E 2 animals. Feeding IRD stimulated bone-formation and inhibited bone-resorption, whereas s.c. or dietary administration of GEN only resulted in a stimulation of bone-formation. The results of the present study indicate that in contrast to s.c. administration, dietary intake of GEN resulted in bone protection without stimulation of UWW. Dietary intake of isoflavones by an IRD also did not result in a stimulation of UWW, yet IRD appeared to be more effective in bone protection than administration of pure GEN. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect