A possible role of prolyl oligopeptidase during Linum usitatissimum (flax) seed development
| Autor: | Laurent Gutierrez, Geneviève Conejero, Jean-Luc Verdeil, Mathieu Castelain, O. Van Wuytswinkel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Linum
DNA Complementary genetic structures Arabidopsis thaliana Molecular Sequence Data Arabidopsis Gene Expression Oligopeptidase F62 - Physiologie végétale - Croissance et développement Plant Science Linum usitatissimum behavioral disciplines and activities Flax Gene expression Expression des gènes Amino Acid Sequence Amplified Fragment Length Polymorphism Analysis Gene Peptide sequence Semence In Situ Hybridization Ecology Evolution Behavior and Systematics biology Reverse Transcriptase Polymerase Chain Reaction urogenital system Serine Endopeptidases food and beverages General Medicine biology.organism_classification Biochemistry Seeds Activité enzymatique Prolyl Oligopeptidases Function (biology) psychological phenomena and processes Développement biologique |
| Zdroj: | Plant Biology |
| Popis: | Involvement of prolyl oligopeptidases (POPs) in the control of several mammalian peptide hormone signalling pathways has been studied extensively in recent years. POPs are ubiquitous enzymes, but little attention has been paid to understanding their function in plants. Using a cDNA-AFLP approach, two flax (Linum usitatissimum) POP ESTs were identified as being specifically expressed in the early stages of flax seed development. This specific expression was confirmed using real time RT-PCR and in situ hybridisation approaches. Seed expression of Arabidopsis POP genes was measured and showed no specificity. Comparison between results obtained with flax and Arabidopsis is discussed in order to address a hypothetic function for POPs during seed formation. These results provide the first insights into POP gene expression and hypothetical function in plants. |
| Databáze: | OpenAIRE |
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