Phytosphingosine-1-phosphate and epidermal growth factor synergistically restore extracellular matrix in human dermal fibroblasts in vitro and in vivo
| Autor: | Ka Ram Kim, Min Jung Kim, Young Min Choi, Hwa Jun Cha, Seung Bin Kwon, Sungkwan An, Kyu Joong Ahn, In-Sook An |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Survival Biology Ligands Extracellular matrix 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Cell Movement Sphingosine In vivo Epidermal growth factor Genetics Humans Phosphorylation Extracellular Signal-Regulated MAP Kinases Cells Cultured Cell Proliferation Wound Healing Epidermal Growth Factor integumentary system Cell growth Dermis General Medicine Fibroblasts In vitro Extracellular Matrix Cell biology ErbB Receptors Blot 030104 developmental biology Wound healing Proto-Oncogene Proteins c-akt A431 cells |
| Zdroj: | International Journal of Molecular Medicine. 39:741-748 |
| ISSN: | 1791-244X 1107-3756 |
| DOI: | 10.3892/ijmm.2017.2866 |
| Popis: | Phytosphingosine-1-phosphate (PhS1P), which is found in plants and fungi, is generated by the phosphorylation of phytosphingosine and is structurally similar to molecules that promote cellular growth and proliferation. The aim of this study was to ascertain whether PhS1P displays synergistic effects together with epidermal growth factor (EGF), which is also critical for activating proliferation, migration and survival pathways. We utilized cultured human dermal fibroblasts (HDFs) and a number of assays, including western blotting, cell migration assays, quantitative (real-time) PCR, and viability assays. We found that PhS1P promoted the activity of EGF in vitro. We then conducted a clinical trial in females over 35 years of age, with visible signs of skin aging. By evaluating skin hydration, dermal density and thickness, length of fine wrinkles, and skin elasticity, we verified the clinical efficacy of a combined treatment of PhS1P and EGF in vivo. On the whole, our data suggest that PhS1P displays a synergistic anti-aging effect together with EGF, both in vitro and in vivo. |
| Databáze: | OpenAIRE |
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