Studies on the Antigenic Structure of Japanese Encephalitis Virus Using Monoclonal Antibodies
| Autor: | Yuzuru Kobayashi, Tetsuzo Yamauchi, Hitoshi Hasegawa |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Hemagglutination
medicine.drug_class Immunology Antibodies Viral Monoclonal antibody Binding Competitive Microbiology Virus Epitope Epitopes Antigen Antibody Specificity Neutralization Tests Virology medicine Antigens Viral Hemagglutination Viral Encephalitis Virus Japanese biology Virion Antibodies Monoclonal Hemagglutination Inhibition Tests Japanese encephalitis medicine.disease biology.organism_classification Molecular biology Flavivirus biology.protein Antibody |
| Zdroj: | Microbiology and Immunology. 29:1069-1082 |
| ISSN: | 0385-5600 |
| DOI: | 10.1111/j.1348-0421.1985.tb00897.x |
| Popis: | The antigenic relationships among 11 strains of Japanese encephalitis (JE) virus were analyzed by using monoclonal antibodies (NARMA) against the Nakayama-RFVL strain in hemagglutination-inhibition (HI) and neutralization (Nt) tests. Of the 14 JE virus-specific HI antibodies, all except NARMA 5 showed Nt reactivity with the homologous strain. The HI and Nt titers of these antibodies were not parallel. The 14 antibodies included the following characteristic antibodies: NARMA 3 is a species-specific antibody with HI and Nt reactivities against JE virus, NARMA 13 is a species-specific HI antibody, NARMA 6 is a Nakayama strain-specific antibody with HI and Nt reactivities, and NARMA 5 is a Nakayama strain-specific HI antibody. The 11 strains of JE virus were divided into four major antigenic groups. However, slight antigenic differences were found among some strains of the same group. Furthermore, competitive binding assays were performed to determine the distribution of antigenic determinants by enzyme-linked immunosorbent assay. The results suggest the existence of at least five HI sites on the JE virus virion, and indicate that the JE species-specific HI site and the flavivirus genus-specific HI site are topologically distinct. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text