Evaluation of the eazyplex MRSA assay for the rapid detection of Staphylococcus aureus in pleural and synovial fluid

Autor: Carmen Muñoz-Almagro, Desiree Henares, Pedro Brotons, X. Buyse, I. Latorre, H.D. de Paz
Rok vydání: 2017
Předmět:
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine
Microbiology (medical)
Staphylococcus aureus
medicine.medical_specialty
Pathology
Microbiological culture
Adolescent
eazyplex MRSA
030106 microbiology
Loop-mediated isothermal amplification
MRSA
MSSA
medicine.disease_cause
Sensitivity and Specificity
Rapid detection
Gastroenterology
lcsh:Infectious and parasitic diseases
03 medical and health sciences
Internal medicine
Synovial Fluid
Humans
Medicine
Synovial fluid
lcsh:RC109-216
Prospective Studies
DNA Primers
Pleural Cavity
business.industry
General Medicine
Staphylococcal Infections
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
medicine.disease
Empyema
Pneumonia
030104 developmental biology
Infectious Diseases
Spain
Performance evaluation
Pleural fluid
Methicillin Resistance
business
Nucleic Acid Amplification Techniques
Pleural and synovial fluid
Zdroj: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
instname
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
r-FSJD: Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
Fundació Sant Joan de Déu
International Journal of Infectious Diseases
r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol
International Journal of Infectious Diseases, Vol 59, Iss C, Pp 65-68 (2017)
ISSN: 1201-9712
1878-3511
DOI: 10.1016/j.ijid.2017.04.013
Popis: Objective: Loop-mediated isothermal amplification (LAMP) is a simple and sensitive technique for rapid microbiological diagnosis. The aim of this study was to evaluate the analytical and diagnostic performance of the LAMP eazyplex MRSA test for the direct detection and differentiation of methicillin-susceptible (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) in synovial/pleural fluid. Methods: Analytical validation included the determination of the limit of detection (LoD) and analytical specificity of the eazyplex MRSA test. A diagnostic evaluation of the eazyplex test against bacterial culture was performed on routine pleural/synovial samples collected prospectively from patients aged less than 18 years with complicated pneumonia with empyema or arthritis admitted to the Children's Hospital Sant Joan de Deu in Barcelona, Spain, between April 2015 and May 2016. Results: The new system appropriately detected a quality control panel of clinical samples with DNA of MSSA, MRSA, and other pathogens. The LoD was established at 6.4 x 10(3) CFU/ml for S. aureus and 1.0 x 10(4) CFU/ml for MRSA. Diagnostic validation of the eazyplex MRSA assay was performed on 51 prospective clinical invasive samples, resulting in sensitivity and specificity values of 83.3% and 97.8%, respectively, for S. aureus detection. The mean turnaround time was 70 min. Conclusions: The eazyplex MRSA assay was found to be a useful test for the rapid detection of S. aureus in invasive samples such as pleural/synovial fluid. (C) 2017 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
Databáze: OpenAIRE
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