Bulges in left-handed G-quadruplexes
| Autor: | Anh Tuân Phan, Khac Huy Ngo, Yves Mechulam, Fernaldo Richtia Winnerdy, Blaž Bakalar, Arijit Maity, Emmanuelle Schmitt, Poulomi Das |
|---|---|
| Přispěvatelé: | Nanyang Technological University [Singapour], Laboratoire de Biologie Structurale de la Cellule (BIOC), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), School of Physical and Mathematical Sciences, NTU Institute of Structural Biology |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Models
Molecular MESH: DNA / chemistry AcademicSubjects/SCI00010 Computational biology Biology 010402 general chemistry Left handedness G-quadruplex Crystallography X-Ray 01 natural sciences 03 medical and health sciences Structural Biology MESH: Nuclear Magnetic Resonance Biomolecular Genetics Nucleotide Motifs Nuclear Magnetic Resonance Biomolecular 030304 developmental biology Left handed 0303 health sciences Crystallography Right handed [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] MESH: G-Quadruplexes Biological sciences [Science] DNA MESH: Crystallography X-Ray Solution structure 0104 chemical sciences G-Quadruplexes MESH: Nucleotide Motifs X-Ray MESH: Sugars / chemistry Sugars MESH: Models Molecular |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 2021, 49 (3), pp.1724-1736. ⟨10.1093/nar/gkaa1259⟩ |
| ISSN: | 0305-1048 1362-4962 |
| DOI: | 10.1093/nar/gkaa1259⟩ |
| Popis: | G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures. Ministry of Education (MOE) Nanyang Technological University National Research Foundation (NRF) Published version Singapore National Research Foundation Investigatorship [NRF-NRFI2017-09]; Singapore Ministry of Education Academic Research Fund Tier 2 [MOE2015-T2- 1092]; Nanyang Technological University (NTU Singapore) grants (to A.T.P.). Funding for open access charge: Nanyang Technological University. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|