The NAC transcription factor ATAF2 promotes ethylene biosynthesis and response in Arabidopsis thaliana seedlings

Autor:	Hao Peng, Jessica Phung, Evan C. Stowe, Amit Dhingra, Michael M. Neff
Rok vydání:	2022
Předmět:	Repressor Proteins Arabidopsis Proteins Gene Expression Regulation Plant Seedlings Structural Biology Arabidopsis Genetics Biophysics Cell Biology Ethylenes Molecular Biology Biochemistry Transcription Factors
Zdroj:	FEBS Letters. 596:1586-1599
ISSN:	1873-3468 0014-5793
Popis:	Arabidopsis thaliana activating factor 2 (ATAF2) plays extensive regulatory roles in pathogenesis, seedling development, and stress responses. Here, we performed transcriptome analysis on ATAF2 loss- and gain-of-function mutants to identify differentially expressed genes (DEGs). Gene ontology analyses on DEGs reveal that ATAF2 enhances seedling responses to multiple hormone and stress signals. In particular, our transcriptome analysis suggests that ATAF2 promotes ethylene biosynthesis and responses via activating relevant genes. This novel role of ATAF2 was further demonstrated by using multiple ATAF2-null and overexpression lines for reverse transcription quantitative PCR verification, ethylene production measurements, and assays of seedlings growth responses to the ethylene immediate biosynthetic precursor 1-aminocyclopropane-1-carboxylic acid (ACC). ACC suppresses ATAF2 expression to form a negative feedback regulation loop.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9e479f736289aa60d297b5e267117ab https://doi.org/10.1002/1873-3468.14317 Zobrazit plný text záznamu Plný text