Reconstruction of an Active Integron Recombination Site after Integration of a Gene Cassette at a Secondary Site
| Autor: | Heidi Segal, M. Victoria Francia, Juan M. García Lobo, Gay Elisha |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Recombination
Genetic Pharmacology Genetics Secondary site Binding Sites Acinetobacter Integrases Restriction Mapping Genetic transfer Biology Integron Molecular biology Integrase Infectious Diseases Gene cassette Plasmid Bacterial Proteins Mechanisms of Resistance biology.protein Pharmacology (medical) Gene Recombination |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 43:2538-2541 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.43.10.2538 |
| Popis: | As the site of insertion of the aadB gene cassette on pRAY, from a clinical isolate of Acinetobacter , is almost identical to the preferred site on integrons, the composite 59-base element (59-BE) associated with this cassette is potentially recombinationally active. By using a conduction assay to quantitate site activity, the 59-BE was recognized by integrase with high frequency, indicating that the composite site is recombinationally active. |
| Databáze: | OpenAIRE |
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