Profiling the expression of fatty acid-binding proteins and fatty acid transporters in mouse microglia and assessing their role in docosahexaenoic acid-d5 uptake
| Autor: | Yijun Pan, Yi Ling Low, J.L. Short, J.A. Nicolazzo |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Docosahexaenoic Acids Clinical Biochemistry 030209 endocrinology & metabolism Fatty Acid-Binding Proteins Fatty acid-binding protein Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Gene silencing chemistry.chemical_classification Messenger RNA 030109 nutrition & dietetics Microglia Fatty Acid Transport Proteins Chemistry food and beverages Fatty acid Transporter Cell Biology Deuterium Neoplasm Proteins medicine.anatomical_structure Biochemistry Blood-Brain Barrier Docosahexaenoic acid lipids (amino acids peptides and proteins) Fatty Acid Binding Protein 3 |
| Zdroj: | Prostaglandins, Leukotrienes and Essential Fatty Acids. 171:102303 |
| ISSN: | 0952-3278 |
| DOI: | 10.1016/j.plefa.2021.102303 |
| Popis: | While the processes governing docosahexaenoic acid (DHA) trafficking across the blood-brain barrier have been elucidated, factors governing DHA uptake into microglia, an essential step for this fatty acid to exert its anti-inflammatory effects, are unknown. This study assessed the mRNA and protein expression of fatty acid-binding proteins (FABPs) and fatty acid transport proteins (FATPs) in mouse BV-2 cells and their mRNA expression in primary mouse microglia. The microglial uptake of DHA-d5, a surrogate of DHA, was assessed by LC-MS/MS following interventions including temperature reduction, silencing of various FABP isoforms, competition with DHA, and metabolic inhibition. It was found that DHA-d5 uptake at 4°C was 39.6% lower than at 37°C, suggesting that microglial uptake of DHA-d5 likely involves passive and/or active uptake mechanisms. Of all FABP and FATP isoforms probed, only FABP3, FABP4, FABP5, FATP1, and FATP4 were expressed at both the mRNA and protein level. Silencing of FABP3, FABP4, and FABP5 resulted in no change in cellular DHA-d5 uptake, nor did concomitant DHA administration or the presence of 0.1% sodium azide/50 mM 2-deoxy-D-glucose. This study is the first to identify the presence of FABPs and FATPs in mouse microglia, albeit these proteins are not involved in the microglial uptake of DHA-d5. |
| Databáze: | OpenAIRE |
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