Transcriptome of the floral transition in Rosa chinensis 'Old Blush'

Autor: Ni Zhen, Qixiang Zhang, Huihua Wan, Xuelian Guo, Jiongrui Tan, Le Luo, Chao Yu, Huitang Pan, Tingliang Xu
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
Circadian clock
Flowers
Biology
Rosa
01 natural sciences
Models
Biological
Transcriptome
03 medical and health sciences
Plant Growth Regulators
Auxin
Gene Expression Regulation
Plant
Hormone signaling
Gene expression
Botany
Genetics
Cluster Analysis
Gene
reproductive and urinary physiology
chemistry.chemical_classification
Floral transition
Gene Expression Profiling
digestive
oral
and skin physiology
fungi
food and beverages
Computational Biology
Molecular Sequence Annotation
Meristem
Sugar signaling
030104 developmental biology
Gene Ontology
chemistry
Recurrent flowering
Differentially expressed genes
Carbohydrate Metabolism
Gibberellin
Function (biology)
Metabolic Networks and Pathways
010606 plant biology & botany
Biotechnology
Research Article
Zdroj: BMC Genomics
ISSN: 1471-2164
Popis: Background The floral transition plays a vital role in the life of ornamental plants. Despite progress in model plants, the molecular mechanisms of flowering regulation remain unknown in perennial plants. Rosa chinensis ‘Old Blush’ is a unique plant that can flower continuously year-round. In this study, gene expression profiles associated with the flowering transition were comprehensively analyzed during floral transition in the rose. Results According to the transcriptomic profiles, 85,663 unigenes and 1,637 differentially expressed genes (DEGs) were identified, among which 32 unigenes were involved in the circadian clock, sugar metabolism, hormone, and autonomous pathways. A hypothetical model for the regulation of floral transition was proposed in which the candidate genes function synergistically the floral transition process. Hormone contents and biosynthesis and metabolism genes fluctuated during the rose floral transition process. Gibberellins (GAs) inhibited rose floral transition, the content of GAs gradually decreased and GA2ox and SCL13 were upregulated from vegetative (VM) meristem to floral meristem (FM). Auxin plays an affirmative part in mediating floral transition, auxin content and auxin-related gene expression levels were gradually upregulated during the floral transition of the rose. However, ABA content and ABA signal genes were gradually downregulated, suggesting that ABA passively regulates the rose floral transition by participating in sugar signaling. Furthermore, sugar content and sugar metabolism genes increased during floral transition in the rose, which may be a further florigenic signal that activates floral transition. Additionally, FRI, FY, DRM1, ELIP, COP1, CO, and COL16 are involved in the circadian clock and autonomous pathway, respectively, and they play a positively activating role in regulating floral transition. Overall, physiological changes associated with genes involved in the circadian clock or autonomous pathway collectively regulated the rose floral transition. Conclusions Our results summarize a valuable collective of gene expression profiles characterizing the rose floral transition. The DEGs are candidates for functional analyses of genes affecting the floral transition in the rose, which is a precious resource that reveals the molecular mechanism of mediating floral transition in other perennial plants. Electronic supplementary material The online version of this article (doi:10.1186/s12864-017-3584-y) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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