Rapid typing of truffle mycorrhizal roots by PCR amplification of the ribosomal DNA spacers
| Autor: | Paola Bonfante, F. Meotto, C. Nosenzo, Antonietta Mello |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Truffle
biology fungi food and beverages Plant Science General Medicine biology.organism_classification law.invention Ectomycorrhiza law Botany Genetics Restriction fragment length polymorphism Mycorrhiza Internal transcribed spacer Molecular Biology Ribosomal DNA Ecology Evolution Behavior and Systematics Mycelium Polymerase chain reaction |
| Popis: | DNA analyses were developed to type mycorrhizas of two Tuber species of commercial value (T. melanosporum, T. borchii) and a competitive fungus (Sphaerosporella brunnea) which forms ectomycorrhizas with plants usually considered hosts for truffles. Polymerase chain reaction (PCR) amplification of DNA isolated from fruitbodies, mycelia, mycorrhizas and leaves of host plants, was performed with a primer pair for an internal transcribed spacer ITS1-4. ITS amplification followed by restriction fragment length polymorphism (RFLP) analysis of the amplified products clearly distinguished the two Tuber species at the fruitbody, mycorrhiza and mycelium levels. |
| Databáze: | OpenAIRE |
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